IL1R1 antibody

Catalog No. ABIN967343

Product Details anti-IL1R1 Antibody

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: BD Pharmingen™

Characteristics: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: HIL1R-M1

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA Capture: The purified HIL1R-M1 antibody (ABIN967343) is useful as a capture antibody for a sandwich ELISA for measuring human IL-1 receptor I protein levels. Purified HIL1R-M1 antibody can be paired with a biotinylated detecting antibody, with recombinant soluble human IL-1 receptor type I as the standard. Purified HIL1R-M1 antibody should be titrated between 1 - 4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human IL-1 receptor type I ranging from 3000 to 20 pg/ml are recommended for inclusion in each ELISA plate.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: sCD14, CD40, CD40L, IL-1Ra, IL-1RII, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, LT-alpha, sTNFRI, sTNFRII, IFNgamma, TRAIL, GM-CSF, TGFbeta, Trx Western Blot: The purified HIL1R-M1.1 antibody (ABIN967343) has been reported to be useful for Western blotting, however, the antibody is not routinely tested for this application.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL1R1 antibody (ABIN967343)

Sims: "CD121a." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 2, pp. 133-5, (2000) (PubMed).

Sims, Painter, Gow: "Genomic organization of the type I and type II IL-1 receptors." in: Cytokine, Vol. 7, Issue 6, pp. 483-90, (1996) (PubMed).

Sims, Giri, Dower: "The two interleukin-1 receptors play different roles in IL-1 actions." in: Clinical immunology and immunopathology, Vol. 72, Issue 1, pp. 9-14, (1994) (PubMed).

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: CD121a (IL1R1 Products)

Synonyms: CD121A antibody, D2S1473 antibody, IL-1R-alpha antibody, IL1R antibody, IL1RA antibody, P80 antibody, CD121a antibody, CD121b antibody, IL-iR antibody, Il1r-1 antibody, DER4 antibody, Fit-1 antibody, Ly84 antibody, ST2L antibody, St2 antibody, St2-rs1 antibody, T1 antibody, T1/ST2 antibody, interleukin 1 receptor type 1 antibody, interleukin 1 receptor, type I antibody, interleukin 1 receptor-like 1 antibody, IL1R1 antibody, Il1r1 antibody, Il1rl1 antibody

Background: The HIL1R-M1 antibody reacts with recombinant and natural soluble CD121a which is also known as human IL-1 receptor type I proteins. Human IL-1 receptor type I serves as the ligand-binding chain of the IL-1 heterodimer complex. When produced by proteolytic cleavage, soluble forms of the IL-1R type I are found the circulation of healthy individuals and in excessive levels during inflammatory diseases. The human IL-1 receptor type I is also expressed predominantly by T lymphocytes, fibroblasts, and endothelial cells.
Synonyms: IL-1 Receptor Type 1

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints