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Caspase 12 (CASP12) antibody

Details for Product No. ABIN967345
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Antigen
Synonyms Caspase-12
Reactivity
Mouse (Murine)
(92), (91), (68)
Host
Rabbit
(118), (9), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB)
(107), (55), (47), (38), (20), (14), (11), (8), (5), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN967345
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Immunogen Murine Caspase-12 peptide
Isotype IgG
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Components 1) 51-16586N: Mouse spleen control lysate.
Quantity: 50 µg (1 ea).
Clone: Polyclonal.
Immunogen: Murine Caspase-12 peptide aa. 2-17.
Isotype: Rabbit Ig.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

2) 51-8104KC: Purified Rabbit Anti-Caspase-1.
Quantity: 100 µl (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide).
Alternative Name Caspase-12
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor forms of caspases are composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation, and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a alpha2beta2 tetramer to form the active enzyme. To date, approximately 14 caspases have been identified in mammals. Caspases can be divided into three groups based upon structural differences and substrate preferences. These include apoptotic initiators (caspase-2, -8, -9, and -10), apoptotic executioners (caspase-3, -6, and -7), and cytokine processors (caspase-1, -4, -5, -13, murine caspase-11, -12, and -14). Caspase-12 has been cloned from the mouse, and based upon sequence homology to other murine caspases (mCASP) can be divided into a subfamily including mCASP1 and -11. The tissue distribution of mCASP-12 was examined by Northern blot analysis and found to be expressed in most tissues, but was highest in skeletal, muscle, and lung tissue. Caspase-12 has been reported to be activated when the endoplasmic reticulum (ER) undergoes stress, and a participant in ER stress-induced apoptosis pathway. Mice deficient for caspase-12 do not undergo ER stress-induced apoptosis, but their cells are capable of undergoing programmed cell death induced by other stimuli. Murine caspase-12 migrates at ~55 kDa in SDS-PAGE. The antibodies recognize human, mouse, and rat caspase-12. A synthetic peptide corresponding to amino acids 2-17 of murine caspase-12 was used as the immunogen.
Research Area Apoptosis/Necrosis
Application Notes Applications include western blot analysis (1:1000-1:4000). Mouse spleen control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C). Additional mouse spleen control lysate (ABIN968544) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN967390, ABIN968544

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Supplier Images
anti-Caspase 12 (CASP12) antibody Western blot analysis of caspase-12. Lysate from mouse spleen was probed with anti-caspase-12 (ABIN967345) at dilutions of 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lane 3). Caspase-12 is identified as a band of ~55 kDa.
Product cited in: Van de Craen, Vandenabeele, Declercq et al.: "Characterization of seven murine caspase family members." in: FEBS letters, Vol. 403, Issue 1, pp. 61-9, 1997 (PubMed).

Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).

Nakagawa, Zhu, Morishima et al.: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).

Yoneda, Imaizumi, Oono et al.: "Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 13935-40, 2001 (PubMed).

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