You are viewing an incomplete version of our website. Please click to reload the website as full version.

Spleen tyrosine Kinase (SYK) (AA 314-339) antibody

Details for Product No. ABIN967351, Supplier: Log in to see
Antigen
  • p72-Syk
  • Sykb
  • p72syk
Alternatives
anti-Human Spleen tyrosine Kinase antibody for Immunocytochemistry
Epitope
AA 314-339
49
33
30
22
21
17
16
15
15
13
11
10
10
9
8
7
7
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
345
133
110
9
7
5
5
5
2
1
1
Host
Mouse
254
85
19
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
13
12
11
8
8
6
3
3
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Western Blotting (WB)
265
134
118
58
56
45
24
22
14
7
4
4
3
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Brand BD Pharmingen™
Immunogen Human Syk peptide aa. 314-339
Clone 4D10
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Syk (SYK Antibody Abstract)
Background Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).
Research Area Immunology, Innate Immunity, Tyrosine Kinases, Signaling
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Buffer SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Spleen tyrosine Kinase (SYK) (AA 314-339) antibody (ABIN967351) Western blot analysis of Syk. Lysate from EB1 cells was probed with anti-Syk (clone 4...
Product cited in: Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001 (PubMed).

Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Chu, van Oers, Malissen et al.: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, 1999 (PubMed).

Costello, Turner, Walters et al.: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." in: Oncogene, Vol. 13, Issue 12, pp. 2595-605, 1997 (PubMed).