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NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 105-291)

Details for Product anti-NFKBIA Antibody No. ABIN967354, Supplier: Login to see
Antigen
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
Alternatives
anti-Human NFKBIA antibody for Western Blotting
Epitope
AA 105-291
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Reactivity
Human
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This NFKBIA antibody is un-conjugated
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Application
Immunoprecipitation (IP), Western Blotting (WB)
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Supplier
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Brand BD Pharmingen™
Immunogen Human IkappaBalpha aa. 105-291 tagged fusion protein
Clone 6A920
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Ikappa B alpha (NFKBIA Antibody Abstract)
Background NF-kappaB is a transcription factor which is a member of the mammalian NF-kappaB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. NF-kappaB is normally found in the cytoplasm and remains in an inactive state by its association with an inhibitory protein, IkappaB. Stimulation of NF-kappaB by a variety of inducers causes the degradation of IkappaBs and translocation of NF-kappaB to the nucleus and activation of the target gene. IkappaBalpha is a member of the IkappaB family of proteins, including IkappaBbeta, IkappaBgamma, Bcl-3, and the precursors of NF-kappaB1 (p105), and NF-kappaB2 (p100). IkappaBalpha is the best characterized member of the family and has been shown to contain three different structural domains: an N-terminal region, an amino acid internal region containing ankyrin repeats, and a C-terminal region containing a PEST domain. In resting cells, IkappaBalpha binds to and maintains NF-kappaB in the cytoplasm by blocking the nuclear localization sequences of NF-kappaB. In response to an extracellular signal, IkappaBalpha is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-kappaB to translocate to the nucleus. Once in the nucleus, NF-kappaB can induce the transcription of IkappaBalpha thereby renewing the cycle so that IkappaBalpha can form a complex with NF-kappaB and maintain it in its cytoplasmic location. IkappaBalpha -/- mice have been shown to die soon after birth and show an increased level NF-kappaB activity. Furthermore, in Hodgkin's lymphoma (HL) a high constitutive level of NF-kappaB has been reported in samples in which clonal deleterious mutations were detected in the IkappaBalpha plays in the pathogenic process which leads to HL remains to be elucidated. IkappaBalpha migrates at ~40 kDa in SDS/PAGE, while the deduced molecular weight based upon its cDNA sequence is ~36 kDa (SWISS PROT Accession number P25963).
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Store the antibody at 4°C. A-431 cell lysate is provided as a positive control (store lysate at -20°C). Additional A-431 control lysate (ABIN968533) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN968533

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the antibody at 4°C. Store lysate at -20°C.
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 105-291) (ABIN967354) Western blot analysis of IkappaBalpha. Lysate from A-431 cells was probed with anti-h...
Product cited in: Karin, Ben-Neriah: "Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity." in: Annual review of immunology, Vol. 18, Issue 8, pp. 621-63, 2000 (PubMed).

Jungnickel, Staratschek-Jox, Bräuninger et al.: "Clonal deleterious mutations in the IkappaBalpha gene in the malignant cells in Hodgkin's lymphoma." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 395-402, 2000 (PubMed).

Klement, Rice, Car et al.: "IkappaBalpha deficiency results in a sustained NF-kappaB response and severe widespread dermatitis in mice." in: Molecular and cellular biology, Vol. 16, Issue 5, pp. 2341-9, 1996 (PubMed).

Beg, Sha, Bronson et al.: "Constitutive NF-kappa B activation, enhanced granulopoiesis, and neonatal lethality in I kappa B alpha-deficient mice." in: Genes & development, Vol. 9, Issue 22, pp. 2736-46, 1995 (PubMed).

Background publications Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).

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