His Tag antibody (HRP)

Details for Product No. ABIN967361, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Mouse
434
184
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
58
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
554
386
167
107
99
77
69
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Sequence HHHHHH
Clone F24-796
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. ProClin is a trademark of Rohm and Haas Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name 6xHis
Target Type Tag
Background The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest. This antibody is routinely tested by western blot analysis.
Molecular Weight 31 kDa
Application Notes The size of the band observed will vary according to the size of the recombinant protein. In the example shown, the lysate contained a recombinant protein of ~31 kDa and the 6x His tag does not appreciably change the observed molecular weight.
The size of the band observed will vary according to the size of the recombinant protein. In the example shown, the lysate contained a recombinant protein of ~31 kDa and the 6xHis tag does not appreciably change the observed molecular weight.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution containing BSA
Preservative ProClin
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (HRP) (ABIN967361) Western blot analysis. A lysate from an E. coli-produced DsRed-6xHis recombinant prot...
Product cited in: Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, 1988 (PubMed).

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