Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

His Tag antibody

WB, IP Host: Mouse Monoclonal F24-796 unconjugated
Catalog No. ABIN967362
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 93
    • 56
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    Mouse
    Clonality
    • 106
    • 69
    • 10
    Monoclonal
    Conjugate
    • 91
    • 22
    • 14
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This His Tag antibody is un-conjugated
    Application
    • 153
    • 103
    • 38
    • 27
    • 25
    • 17
    • 14
    • 14
    • 14
    • 13
    • 13
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Brand
    BD Pharmingen™
    Sequence
    HHHHHH
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    F24-796
    Isotype
    IgG2b
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    An E. coli produced recombinant 6xHis control lysate is provided as a positive control. Store lysates at -20°C.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, (1988) (PubMed).

  • Target
    His Tag
    Alternative Name
    6xHis (His Tag Products)
    Target Type
    Tag
    Background
    The engineering of a short epitope tag to a recombinant protein provides a very convenient method for detecting and isolating the protein of interest. For example, the placement of a series of 6 consecutive histidine residues that are incorporated at either end of the recombinant fusion protein facilitates the purification of that protein. Purification can be easily accomplished by metal chelate affinity chromatography using a metal resin column. The 6xHis monoclonal antibody can be used to detect the epitope-tagged protein of interest.
You are here:
Support