Integrin beta 1 (ITGB1) antibody

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Antigen
  • cd29
  • fnrb
  • gpiia
  • itgb1
  • mdf2
  • msk12
  • vla-beta
  • vlab
  • CD29
  • 4633401G24Rik
  • AA409975
  • AA960159
  • ENSMUSG00000051907
  • Fnrb
  • Gm9863
  • gpIIa
  • FNRB
  • GPIIA
  • MDF2
  • MSK12
  • VLA-BETA
  • VLAB
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
  • integrin beta 1 (fibronectin receptor beta)
  • integrin, beta 1
  • itgb1-b
  • ITGB1
  • Itgb1
Alternatives
anti-Mouse (Murine) Integrin beta 1 antibody for Blocking Peptide
Reactivity
Mouse (Murine)
737
276
162
89
69
33
22
18
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12
6
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1
Host
Rat
553
203
73
50
38
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
77
72
50
31
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1
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1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
614
443
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191
143
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109
88
54
19
13
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3
3
2
2
2
1
1
1
1
1
Options
Supplier
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Brand BD Pharmingen™
Immunogen Mouse endothelial cell line
Clone 9EG7
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD29 (ITGB1 Antibody Abstract)
Background The 9EG7 antibody reacts with the 130-kDa integrin beta1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin alpha chains. With alpha1 through alpha6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with alphav (CD51), it forms alphavbeta1 integrin. It also associates with the integrin alpha7 alpha8 and alpha9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. 9EG7 mAb has been shown to inhibit both the alpha6beta1-mediated binding of lymphocytes to endothelial cells and the adhesion mediated by activated, but not unactivated, alpha4beta1-integrin. The source of the immunogen was mouse lymph node-derived endothelial cell line TME.
Synonyms: Integrin beta1 chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex
Application Notes For IHC, we recommend the use of purified 9EG7 mAb in our special formulation for immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Product cited in: Yashiro, Tai, Toyo-oka, Park, Abe, Hamaoka, Kobayashi, Neben, Fujiwara: "A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules." in: European journal of immunology, Vol. 28, Issue 3, pp. 926-35, 1998 (PubMed).

Driessens, Van Rijthoven, Kemperman, Roos: "Adhesion of lymphoma cells to fibronectin: differential use of alpha 4 beta 1 and alpha 5 beta 1 integrins and stimulation by the 9EG7 mAb against the murine beta 1 integrin subunit." in: Cell adhesion and communication, Vol. 3, Issue 4, pp. 327-36, 1996 (PubMed).

Milner, Edwards, Streuli, Ffrench-Constant: "A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 22, pp. 7240-52, 1996 (PubMed).

Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, 1996 (PubMed).

Kassner, Kawaguchi, Hemler: "Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion." in: The Journal of biological chemistry, Vol. 269, Issue 31, pp. 19859-67, 1994 (PubMed).

Lenter, Vestweber: "The integrin chains beta 1 and alpha 6 associate with the chaperone calnexin prior to integrin assembly." in: The Journal of biological chemistry, Vol. 269, Issue 16, pp. 12263-8, 1994 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Background publications Lenter, Uhlig, Hamann, Jenö, Imhof, Vestweber: "A monoclonal antibody against an activation epitope on mouse integrin chain beta 1 blocks adhesion of lymphocytes to the endothelial integrin alpha 6 beta 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9051-5, 1993 (PubMed).