Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) antibody

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Antigen
  • MADCAM1
  • AV211525
  • MACAM1
  • mucosal vascular addressin cell adhesion molecule 1
  • MADCAM1
  • Madcam1
  • MADCAM-1
Reactivity
Mouse (Murine)
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2
Host
Rat
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1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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4
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2
2
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2
2
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2
2
2
1
1
1
Application
Blocking Reagent (BR), Immunoaffinity Chromatography (IAC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
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103
45
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10
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2
2
2
2
2
1
1
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Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.
Clone MECA-367
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MAdCAM-1 (MADCAM1 Antibody Abstract)
Background The MECA-367 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-gamma can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin alpha4beta7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV in vivo. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.
Application Notes For IHC, we recommend the use of purified MECA-367 mAb in our special formulation for immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Background publications Picarella, Hurlbut, Rottman, Shi, Butcher, Ringler: "Monoclonal antibodies specific for beta 7 integrin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) reduce inflammation in the colon of scid mice reconstituted with CD45RBhigh CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 5, pp. 2099-106, 1997 (PubMed).

Mebius, Streeter, Michie, Butcher, Weissman: "A developmental switch in lymphocyte homing receptor and endothelial vascular addressin expression regulates lymphocyte homing and permits CD4+ CD3- cells to colonize lymph nodes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 11019-24, 1996 (PubMed).

Product cited in: Yang, Sytwu, McDevitt, Michie: "Involvement of beta 7 integrin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the development of diabetes in obese diabetic mice." in: Diabetes, Vol. 46, Issue 10, pp. 1542-7, 1997 (PubMed).

Hänninen, Salmi, Simell, Andrew, Jalkanen: "Recirculation and homing of lymphocyte subsets: dual homing specificity of beta 7-integrin(high)-lymphocytes in nonobese diabetic mice." in: Blood, Vol. 88, Issue 3, pp. 934-44, 1996 (PubMed).

Kraal, Schornagel, Streeter, Holzmann, Butcher: "Expression of the mucosal vascular addressin, MAdCAM-1, on sinus-lining cells in the spleen." in: The American journal of pathology, Vol. 147, Issue 3, pp. 763-71, 1995 (PubMed).

Berg, McEvoy, Berlin, Bargatze, Butcher: "L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, 1994 (PubMed).

Hamann, Andrew, Jablonski-Westrich, Holzmann, Butcher: "Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3282-93, 1994 (PubMed).

Lee, Sarvetnick: "Induction of vascular addressins and adhesion molecules in the pancreas of IFN-gamma transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 9, pp. 4597-603, 1994 (PubMed).

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Briskin, McEvoy, Butcher: "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1." in: Nature, Vol. 363, Issue 6428, pp. 461-4, 1993 (PubMed).

Streeter, Berg, Rouse, Bargatze, Butcher: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." in: Nature, Vol. 331, Issue 6151, pp. 41-6, 1988 (PubMed).

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