Jun Proto-Oncogene (JUN) (C-Term) antibody

Details for Product No. ABIN967394
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Antigen
Synonyms p39, B230310J22Rik, AP1, AP-1, c-Jun, Junc, c-jun, Jun, JUN, fj36h07, zgc:65863, wu:fj36h07
Epitope
C-Term
(71), (60), (55), (46), (37), (37), (29), (19), (13), (10), (9), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(599), (419), (387), (154), (152), (150), (139), (10), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Host
Mouse
(556), (57), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application
Western Blotting (WB), Gel Shift (GS)
(467), (222), (180), (127), (119), (66), (49), (21), (19), (8), (8), (6), (2), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN967394
Quantity 0.1 mg
Price
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Immunogen Recombinant half of c-Jun
Clone G56-206
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Jun
Background The cellular Jun (c-Jun) and its viral counterpart, v-Jun, are efficient transcriptional activators. Both c-Jun and v-Jun are sequence-specific DNA-binding proteins that recognize the same sequence motif, termed TRE (TPA responsive element). c-Jun does not recognize this motif unless it forms a heteromeric complex with c-fos, forming the transcription factor AP1. Jun family members JunB and JunD are 44% and 45% identical to c-Jun on the protein level, respectively. Jun family members are highly conserved between species. There is 98% amino acid identity between human and mouse c-Jun, and 45% between chicken and mouse c-Jun. There is 98% amino acid homology between human and mouse JunB, 95% between human and rat JunB, and 99.5% between mouse and rat JunB. There is 77% amino acid homology between human and mouse JunD and 71% between chicken and mouse JunD. G56-206 recognizes Jun family members including c-Jun, JunB, and JunD. A truncated recombinant protein consisting of the C-terminal half of c-Jun was used as immunogen. This region contains the c-Jun DNA binding domain. The antibody was evaluated by western blot analysis using in vitro translated c-Jun and Cos-7 monkey kidney cells transfected with recombinant c-Jun.
Molecular Weight 39 kDa, (c-Jun), 35 kDa (JunB), 40-50 kDa (JunD)
Application Notes We recommend to use 1-2 µg/ml of antibody concentration for western blot application.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Jun Proto-Oncogene (JUN) (C-Term) antibody Western blot analysis of c-Jun. Lane 1, recombinant c-Jun. Lane 2, BSA (negative control). Lane 3, Cos-7 cell lysate (negative control). Lane 4, Cos-7 lysate from cells which were transiently transfected with c-Jun. Anti-Jun identifies both recombinant c-Jun protein (lane 1) as well as c-Jun which is expressed in Cos-7 cells (lane 4) as an ~39 kDa band.
anti-Jun Proto-Oncogene (JUN) (C-Term) antibody (2) anti-Jun Proto-Oncogene (JUN) (C-Term) antibody (Image 2)
Product cited in: Curran, Bravo, Müller: "Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation." in: Cancer surveys, Vol. 4, Issue 4, pp. 655-81, 1988 (PubMed).

General Angel, Karin: "The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation." in: Biochimica et biophysica acta, Vol. 1072, Issue 2-3, pp. 129-57, 1992 (PubMed).

Hosts (556), (57), (2), (1), (1)
Reactivities (599), (419), (387), (154), (152), (150), (139), (10), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Applications (467), (222), (180), (127), (119), (66), (49), (21), (19), (8), (8), (6), (2), (1), (1), (1)
Conjugates (12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Epitopes (71), (60), (55), (46), (37), (37), (29), (19), (13), (10), (9), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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