Jun Proto-Oncogene (JUN) (C-Term) antibody

Antigen: Jun Proto-Oncogene (JUN)

Synonyms: AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun

Binding Site: C-Term

Clonality: Monoclonal (G56-206)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Gel Shift (GS)

Catalog no.: ABIN967394

Additional Information

Alternative name: Jun

Immunogen: Recombinant half of c-Jun

Format: Liquid

Isotype: IgG1

Clone: G56-206

Description: The cellular Jun (c-Jun) and its viral counterpart, v-Jun, are efficient transcriptional activators. Both c-Jun and v-Jun are sequence-specific DNA-binding proteins that recognize the same sequence motif, termed TRE (TPA responsive element). c-Jun does not recognize this motif unless it forms a heteromeric complex with c-fos, forming the transcription factor AP1. Jun family members JunB and JunD are 44% and 45% identical to c-Jun on the protein level, respectively. Jun family members are highly conserved between species. There is 98% amino acid identity between human and mouse c-Jun, and 45% between chicken and mouse c-Jun. There is 98% amino acid homology between human and mouse JunB, 95% between human and rat JunB, and 99.5% between mouse and rat JunB. There is 77% amino acid homology between human and mouse JunD and 71% between chicken and mouse JunD. G56-206 recognizes Jun family members including c-Jun, JunB, and JunD. A truncated recombinant protein consisting of the C-terminal half of c-Jun was used as immunogen. This region contains the c-Jun DNA binding domain. The antibody was evaluated by western blot analysis using in vitro translated c-Jun and Cos-7 monkey kidney cells transfected with recombinant c-Jun.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 39 kDa, (c-Jun), 35 kDa (JunB), 40-50 kDa (JunD)

Comments:

Related Products: ABIN967389

Application Details

Application Notes: We recommend to use 1-2 µg/ml of antibody concentration for western blot application.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of c-Jun. Lane 1, recombinant c-Jun. Lane 2, BSA (negative control). Lane 3, Cos-7 cell lysate (negative control). Lane 4, Cos-7 lysate from cells which were transiently transfected with c-Jun. Anti-Jun identifies both recombinant c-Jun protein (lane 1) as well as c-Jun which is expressed in Cos-7 cells (lane 4) as an ~39 kDa band.

anti-Jun Proto-Oncogene (JUN) (C-Term) antibody (Image 2)

Publications

Angel, Karin: "The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation." in: Biochimica et biophysica acta, Vol. 1072, Issue 2-3, pp. 129-57, 1992 (PubMed).

Product: Curran, Bravo, Müller: "Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation." in: Cancer surveys, Vol. 4, Issue 4, pp. 655-81, 1988 (PubMed).