Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody

Details for Product No. ABIN967399
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Antigen
Synonyms AW556805, Mcl-1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, TM, bcl2-L-3, mcl1/EAT, mcl-1
Epitope
AA 121-139
(19), (19), (17), (12), (12), (9), (7), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(230), (82), (59), (18), (12), (3), (3), (2), (2), (1)
Host
Rabbit
(163), (64), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(193), (84), (74), (66), (53), (43), (22), (20), (19), (11), (11), (6), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
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Catalog No. ABIN967399
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Immunogen Human Mcl-1 Peptide
Isotype Ig
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
4. Please refer to us for technical protocols.
Purification Serum
Alternative Name Mcl-1
Background Mcl-1 (for myeloid cell leukemia-1) is a member of the Bcl-2 family that was identified by screening of of cDNA libraries derived from differentiating human myeloid leukemia cells. Bcl-2 family members are involved in apoptosis and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax can homodimerize as well as heterodimerize with Bcl-2. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Although its function remains to be fully elucidated, Mcl-1 may, like Bcl-2, be a blocker of cell death. For example, transfection of Mcl-1 into Chinese hamster ovary cells partially blocked Myc-induced apoptosis, and Mcl-1 blocked Bax mediated cell death in a yeast two-hybrid system. The two-hybrid system results suggest that Mcl-1/Bax interations may have functional homology to Bcl-2/Bax interations in blocking Bax-mediated cell death. This polyclonal antibody is not recommended for the identification of mouse Mcl-1 and has been reported not to cross-react with human Bcl-2.
Molecular Weight 39-42 kDa
Research Area Cancer, Apoptosis/Necrosis
Comment

Related Products: ABIN967390

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide, Thimerosal (Merthiolate)
Storage 4 °C
Supplier Images
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody Western blot analysis for Mcl-1. Left figure: In vitro translated human Mcl-1 (lane 1) and a lysate from human tonsil (lane 2) were probed with the polyclonal rabbit anti-human Mcl-1 antibody at a 1:1000 dilution. The lower band below the 39-42 kDa Mcl-1 band observed in lane 1 may represent a degradation product or a translation initiation product from an internal AUG codon.
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody (2) A SK-N-SH cell lysate (Human neuroblastoma, ATCC HTB-11) was probed with the polyclonal rabbit anti-human Mcl-1 antibody at a 1:1000 dilution. Mcl-1 is identified as the band at 39-42 kDa.
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody (3) anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody (Image 3)
General Kozopas, Yang, Buchan et al.: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993 (PubMed).

Krajewski, Bodrug, Krajewska et al.: "Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in vivo." in: The American journal of pathology, Vol. 146, Issue 6, pp. 1309-19, 1995 (PubMed).

Sato, Hanada, Bodrug et al.: "Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 20, pp. 9238-42, 1994 (PubMed).

Reynolds, Yang, Qian et al.: "Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells." in: Cancer research, Vol. 54, Issue 24, pp. 6348-52, 1995 (PubMed).

Krajewski, Bodrug, Gascoyne et al.: "Immunohistochemical analysis of Mcl-1 and Bcl-2 proteins in normal and neoplastic lymph nodes." in: The American journal of pathology, Vol. 145, Issue 3, pp. 515-25, 1994 (PubMed).

Ohta, Iwai, Kasahara et al.: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996 (PubMed).

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