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BCL2-Associated X Protein (BAX) (AA 43-61) antibody

Details for Product No. ABIN967400
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Antigen
Synonyms BCL2L4, BAX-ALPHA, bax, fj16e01, wu:fc50b10, wu:fj16e01, bax-A, xBax, xlbax, BAX
Epitope
AA 43-61
(69), (29), (19), (19), (8), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(315), (151), (145), (51), (51), (49), (12), (4), (2), (2), (1)
Host
Rabbit
(238), (106), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(241), (137), (79), (76), (69), (68), (40), (28), (12), (9), (2), (2), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
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Catalog No. ABIN967400
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human Bax synthetic peptide
Isotype Ig
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Purity Serum
Alternative Name Bax
Background Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax can homodimerize as well as heterodimerize with Bcl-2. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. mRNA and protein studies indicate that Bax is more widely expressed than Bcl-2, suggesting that Bax has other functions besides inhibiting Bcl-2. For example, Bax has been shown to be directly induced by the tumor suppressor p53 in radiation sensitive cells, and thus may play a role in the apoptotic elimination of cells after their exposure to DNA-damaging agents. The Bax gene potentially encodes three different proteins Bax-alpha (21 kD), Bax-beta (24 kD) and Bax-gamma (5 kD). Bax-alpha is the most common transcript. The antibodies recognize human Bax. They do not cross-react with mouse Bax. A synthetic peptide corresponding to amino acids 43-61 of human Bax was used as immunogen. The specificity of the antiserum was verified by immunoprecipitation, western blot analyisis, and immunohistochemisty, as well as by comparisons with preimmune serum and by competition experiments.
Molecular Weight 21-22 kDa
Research Area Cancer, Apoptosis/Necrosis
Application Notes Applications include immunoprecipitation (1 µl/one million cells), western blot analysis (1:1000), and immunohistochemistry (1:1000) of acetone-fixed, frozen tissue sections and of formalin-fixed, paraffin-embedded tissue sections. Normal breast epithelium is suggested as a positive control for immunohistochemistry. MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as positive controls for immunoprecipitation and western blot analysis. In western blots the antiserum detects Bax as a single band, thought to be Bax-alpha, of 21-22 kD in cell lines tested. The antiserum may also recognize Bax-beta.
Comment

Related Products: ABIN967390, ABIN968585

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide, Thimerosal (Merthiolate)
Storage 4 °C
General Krajewski, Blomqvist, Franssila et al.: "Reduced expression of proapoptotic gene BAX is associated with poor response rates to combination chemotherapy and shorter survival in women with metastatic breast adenocarcinoma." in: Cancer research, Vol. 55, Issue 19, pp. 4471-8, 1995 (PubMed).

Krajewski, Krajewska, Shabaik et al.: "Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2." in: The American journal of pathology, Vol. 145, Issue 6, pp. 1323-36, 1995 (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994 (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, 1994 (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Ohta, Iwai, Kasahara et al.: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967400
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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