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Recombination Activating Gene 1 (RAG1) antibody

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Synonyms RAG-1, rag1, RNF74, Rag-1, rag-1, rag1a
(22), (17), (13), (6), (3)
(28), (4)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(13), (11), (10), (3)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Recombinant Human Rag-1
Clone G189-1417
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Rag-1 (RAG1 Antibody Abstract)
Background Rag-1 and Rag-2 are recombination-activating genes expressed in maturing lymphoid cells. Both genes are required for variable-diversity-joining V(D)J DNA recombination at loci for immunoglobulin and T cell receptor genes. Ablation mutation of either Rag-1 or Rag-2 genes results in the lack of recombination in T and B cells. The normally lymphocyte-specific process of V(D)J recombination can be induced in fibroblasts by co-transfection of Rag-1 and Rag-2. Rag-1 and Rag-2 are both normally expressed in bone marrow and thymus, but only Rag-1 is expressed in the central nervous system. Rag-1 migrates at a reduced molecular weight of 105-120 kDa. Clone G189-1417 recognizes human Rag-1. It does not cross-react with Rag-2. It does not appear to recognize mouse Rag-1. Recombinant human Rag-1 was used as immunogen.
Molecular Weight 105-120 kDa

Related Products: ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Recombination Activating Gene 1 (RAG1) antibody (ABIN967402) Western blot analysis of Rag-1. Lysate from Jurkat cells was probed with anti- Rag-1 ...
 image for anti-Recombination Activating Gene 1 (RAG1) antibody (ABIN967402) anti-Recombination Activating Gene 1 (RAG1) antibody (Image 2)
Product cited in: Silver, Spanopoulou, Mulligan et al.: "Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 13, pp. 6100-4, 1993 (PubMed).

Oettinger, Schatz, Gorka et al.: "RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination." in: Science (New York, N.Y.), Vol. 248, Issue 4962, pp. 1517-23, 1990 (PubMed).

Chun, Schatz, Oettinger et al.: "The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system." in: Cell, Vol. 64, Issue 1, pp. 189-200, 1991 (PubMed).

Mombaerts, Iacomini, Johnson et al.: "RAG-1-deficient mice have no mature B and T lymphocytes." in: Cell, Vol. 68, Issue 5, pp. 869-77, 1992 (PubMed).

Shinkai, Rathbun, Lam et al.: "RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement." in: Cell, Vol. 68, Issue 5, pp. 855-67, 1992 (PubMed).

Background publications Leu, Schatz: "rag-1 and rag-2 are components of a high-molecular-weight complex, and association of rag-2 with this complex is rag-1 dependent." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5657-70, 1995 (PubMed).

Catalog No. ABIN967402
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