Recombination Activating Gene 1 (RAG1) antibody

Antigen: Recombination Activating Gene 1 (RAG1)

Synonyms: RNF74, MGC43321, Rag-1, RAG-1, rag-1

Clonality: Monoclonal (G189-1417)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967402

Additional Information

Alternative name: Rag-1

Immunogen: Recombinant Human Rag-1

Format: Liquid

Isotype: IgG1

Clone: G189-1417

Description: Rag-1 and Rag-2 are recombination-activating genes expressed in maturing lymphoid cells. Both genes are required for variable-diversity-joining V(D)J DNA recombination at loci for immunoglobulin and T cell receptor genes. Ablation mutation of either Rag-1 or Rag-2 genes results in the lack of recombination in T and B cells. The normally lymphocyte-specific process of V(D)J recombination can be induced in fibroblasts by co-transfection of Rag-1 and Rag-2. Rag-1 and Rag-2 are both normally expressed in bone marrow and thymus, but only Rag-1 is expressed in the central nervous system. Rag-1 migrates at a reduced molecular weight of 105-120 kDa. Clone G189-1417 recognizes human Rag-1. It does not cross-react with Rag-2. It does not appear to recognize mouse Rag-1. Recombinant human Rag-1 was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 105-120 kDa

Comments:

Related Products: ABIN968537

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of Rag-1. Lysate from Jurkat cells was probed with anti- Rag-1 (clone G189-1417) at concentrations of 2.0, 1.0, and 0.5 myg/ml. The antibody identifies Rag-1 as a ~110 kDa band.

anti-Recombination Activating Gene 1 (RAG1) antibody (Image 2)

Publications

Shinkai, Rathbun, Lam et al.: "RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement." in: Cell, Vol. 68, Issue 5, pp. 855-67, 1992 (PubMed).

Mombaerts, Iacomini, Johnson et al.: "RAG-1-deficient mice have no mature B and T lymphocytes." in: Cell, Vol. 68, Issue 5, pp. 869-77, 1992 (PubMed).

Chun, Schatz, Oettinger et al.: "The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system." in: Cell, Vol. 64, Issue 1, pp. 189-200, 1991 (PubMed).

Oettinger, Schatz, Gorka et al.: "RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination." in: Science (New York, N.Y.), Vol. 248, Issue 4962, pp. 1517-23, 1990 (PubMed).

Leu, Schatz: "rag-1 and rag-2 are components of a high-molecular-weight complex, and association of rag-2 with this complex is rag-1 dependent." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5657-70, 1995 (PubMed).

Silver, Spanopoulou, Mulligan et al.: "Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 13, pp. 6100-4, 1993 (PubMed).