Retinoblastoma 1 antibody (C-Term)

Catalog No. ABIN967408

Product Details anti-Retinoblastoma 1 Antibody

Target: Retinoblastoma 1 (RB1)

Binding Specificity: C-Term

Reactivity: Human, Chicken, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Cross-Reactivity: Chicken, Mink

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant carboxy-terminal truncated Rb protein

Clone: XZ91

Isotype: IgG2a

Application Details

Application Notes: Applications include immunoprecipitation (1-2 µg/one million cells), western blot analysis (2 µg/ml) and immunofluorescence microscopy of cultured cells. Rb migrates as multiple closely-spaced bands between approximately 110-116 kD when sized on denaturing polyacrylamide gels (i.e. by SDS-PAGE). The different bands represent different Rb phosphorylation states, the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Polyacrylamide gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, we recommend a 4 to 20% gradient gel (equal or more than 12 inches long). MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Retinoblastoma 1 antibody (ABIN967408)

Riley, Lee, Lee: "The retinoblastoma protein: more than a tumor suppressor." in: Annual review of cell biology, Vol. 10, pp. 1-29, (1995) (PubMed).

Ewen, Sluss, Whitehouse, Livingston: "TGF beta inhibition of Cdk4 synthesis is linked to cell cycle arrest." in: Cell, Vol. 74, Issue 6, pp. 1009-20, (1993) (PubMed).

Hollingsworth, Chen, Lee: "Integration of cell cycle control with transcriptional regulation by the retinoblastoma protein." in: Current opinion in cell biology, Vol. 5, Issue 2, pp. 194-200, (1993) (PubMed).

Livingston: "Functional analysis of the retinoblastoma gene product and of RB-SV40 T antigen complexes." in: Cancer surveys, Vol. 12, Issue 6, pp. 153-60, (1992) (PubMed).

Dyson, Guida, McCall, Harlow: "Adenovirus E1A makes two distinct contacts with the retinoblastoma protein." in: Journal of virology, Vol. 66, Issue 7, pp. 4606-11, (1992) (PubMed).

Qin, Chittenden, Livingston, Kaelin: "Identification of a growth suppression domain within the retinoblastoma gene product." in: Genes & development, Vol. 6, Issue 6, pp. 953-64, (1992) (PubMed).

Hu, Bautista, Edwards, Defeo-Jones, Jones, Harlow: "Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides." in: Molecular and cellular biology, Vol. 11, Issue 11, pp. 5792-9, (1991) (PubMed).

Dyson, Bernards, Friend, Gooding, Hassell, Major, Pipas, Vandyke, Harlow: "Large T antigens of many polyomaviruses are able to form complexes with the retinoblastoma protein." in: Journal of virology, Vol. 64, Issue 3, pp. 1353-6, (1990) (PubMed).

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb (RB1 Products)

Background: Nur77 (also known as NGF1-B, N10, TISI) is a growth factor-inducible orphan member of the steroid/thyroid hormone receptor superfamily. This superfamily encodes ligand-dependent transcription factors with a centrally located, highly conserved DNA-binding domain containing two zinc-fingers. Although Nur77 binds no known ligand, it is constitutively active when synthesized. Nur77 was originally identified as an immediate-early gene rapidly activated by serum stimulation of quiescent fibroblasts. It has since been shown to be activated by diverse signals including membrane depolarization, nerve growth factor, chemically induced seizures, adrenocorticotrophic hormone (ACTH), pentylene tetrazole, forskolin and cAMP. Nur77, like other immediate-early genes such as c-myc, has also been shown to have a role in apoptosis. Apoptosis is an internal, programmed cell death which takes place during normal development. Nur77 has been demonstrated to be required for in vitro T-cell-receptor (TCR) mediated negative selection. Negative selection, or the clonal deletion of thymocytes, normally occurs by apoptosis following engagement of the TCR. Nur77 is present in high levels in T-cell hybrids and thymocytes undergoing apoptosis, but not in growing T cells or stimulated splenocytes. T-cell hybrids are protected from activation-induced apoptosis by a Nur77 dominant negative mutation. Induction of Nur77 mRNA and cell death by apoptosis following treatment of T-cell hybrids with antibody directed against the TCR has also been shown. Additionally, transfection of the T-cell hybrids with antisense Nur77 protects cells from apoptosis when signaled to die by TCR engagement. Nur77 is a phosphoprotein which migrates on SDS/PAGE gels as diffuse bands between 67 and 88 kDa depending on post-translational modifications. Clone 12.14 recognizes mouse Nur77. A full-length mouse Nur77 fusion protein was used as immunogen.
Synonyms: NGF1-B, N10, TISI

Molecular Weight: 105-116 kDa

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy