C-Fos antibody

Details for Product No. ABIN967415
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Antigen
Synonyms AP-1, C-FOS, p55, c-fos, D12Rfj1, cFos, cFOS, fos
Reactivity
Human, Mouse (Murine)
(300), (191), (180), (54), (42), (30), (25), (2), (1), (1)
Host
Mouse
(271), (35), (13), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)
(223), (129), (81), (70), (44), (14), (12), (12), (11), (8), (7), (3), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967415
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Clone G54-9-9
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Fos
Background The cellular fos protein (c-fos) and its viral counterpart v-fos belong, along with fosB, fra-1, and fra-2, to the fos family of transcription factors. They are related to other transcription factor families such as the jun proteins (e.g., CREB, DBP, and C/EBB) with which they share a conserved basic domain (DNA binding motif) and a leucine zipper domain. Whereas jun proteins can form homodimers that act as transcriptional activators, fos proteins only form heterodimers, preferentially with jun proteins. The transcription factor AP-1, which selectively binds to enchancer elements in the promoter region of a wide variety of genes, is a heterodimer of c-fos and c-jun. Deletion analyses and mutagenesis studies have shown that the leucine zipper region from fos and jun is necessary for selective heterodimer formation. c-fos appears primarily as a 55 kDa protein on SDS-polyacrylamide gels, however, it undergoes post-translational modification and other forms have been described including 57 kDa, 60 kDa, and 62 kDa.
Clone G54-9.9 recognizes mouse and human c-fos as single or multiple bands (55-62 kDa). Reactivity to fos proteins of other species has not yet been tested. Mice were immunized with a fos specific fragment (amino acid residues 73-152) of the FBJ-MSV v-fos protein. The region excludes completely the DNA binding and leucine zipper motifs.
Molecular Weight 55-62 kDa
Application Notes Clone G54-9.9 may be used for western blot analysis (1-2 µg/ml). Please note that c-fos is an unstable protein, distinct bands are only detected in preparations where c-fos has been induced or overexpressed. Other applications include immunoprecipitation (2-4 µg/ml of cell lysate) and gel shift assays, which are not routinely.
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-C-Fos antibody Western blot analysis of c-fos. Lysate from A431 cells were treated with 50 ng/ml of Epidermal Growth Factor (EGF) to upregulate expression of c-fos. Lane 1, unstimulated cells, lane 2, stimulated cells.
anti-C-Fos antibody (2) anti-C-Fos antibody (Image 2)
Product cited in: Curran, Bravo, Müller: "Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation." in: Cancer surveys, Vol. 4, Issue 4, pp. 655-81, 1988 (PubMed).

General OShea, Rutkowski, Kim: "Mechanism of specificity in the Fos-Jun oncoprotein heterodimer." in: Cell, Vol. 68, Issue 4, pp. 699-708, 1992 (PubMed).

Angel, Karin: "The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation." in: Biochimica et biophysica acta, Vol. 1072, Issue 2-3, pp. 129-57, 1992 (PubMed).

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