C-Fos antibody

Antigen: C-Fos

Clonality: Monoclonal (G54-9-9)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)

Catalog no.: ABIN967415

Additional Information

Alternative name: Fos

Format: Liquid

Isotype: IgG1

Clone: G54-9-9

Description: The cellular fos protein (c-fos) and its viral counterpart v-fos belong, along with fosB, fra-1, and fra-2, to the fos family of transcription factors. They are related to other transcription factor families such as the jun proteins (e.g., CREB, DBP, and C/EBB) with which they share a conserved basic domain (DNA binding motif) and a leucine zipper domain. Whereas jun proteins can form homodimers that act as transcriptional activators, fos proteins only form heterodimers, preferentially with jun proteins. The transcription factor AP-1, which selectively binds to enchancer elements in the promoter region of a wide variety of genes, is a heterodimer of c-fos and c-jun. Deletion analyses and mutagenesis studies have shown that the leucine zipper region from fos and jun is necessary for selective heterodimer formation. c-fos appears primarily as a 55 kDa protein on SDS-polyacrylamide gels, however, it undergoes post-translational modification and other forms have been described including 57 kDa, 60 kDa, and 62 kDa.
Clone G54-9.9 recognizes mouse and human c-fos as single or multiple bands (55-62 kDa). Reactivity to fos proteins of other species has not yet been tested. Mice were immunized with a fos specific fragment (amino acid residues 73-152) of the FBJ-MSV v-fos protein. The region excludes completely the DNA binding and leucine zipper motifs.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 55-62 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Application Notes: Clone G54-9.9 may be used for western blot analysis (1-2 µg/ml). Please note that c-fos is an unstable protein, distinct bands are only detected in preparations where c-fos has been induced or overexpressed. Other applications include immunoprecipitation (2-4 µg/ml of cell lysate) and gel shift assays, which are not routinely.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of c-fos. Lysate from A431 cells were treated with 50 ng/ml of Epidermal Growth Factor (EGF) to upregulate expression of c-fos. Lane 1, unstimulated cells, lane 2, stimulated cells.

anti-C-Fos antibody (Image 2)

Publications

OShea, Rutkowski, Kim: "Mechanism of specificity in the Fos-Jun oncoprotein heterodimer." in: Cell, Vol. 68, Issue 4, pp. 699-708, 1992 (PubMed).

Angel, Karin: "The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation." in: Biochimica et biophysica acta, Vol. 1072, Issue 2-3, pp. 129-57, 1992 (PubMed).

Product: Curran, Bravo, Müller: "Transient induction of c-fos and c-myc in an immediate consequence of growth factor stimulation." in: Cancer surveys, Vol. 4, Issue 4, pp. 655-81, 1988 (PubMed).