Images for product: anti-Retinoblastoma 1 (RB1) (AA 514-610), (underphosphorylated) antibody
Selective binding of G99-549 to underphosphorylated Rb. MOLT-4 cells (ATCC CRL-1582, Human T lymphoblast) were labeled with [32P]-orthophosphate and immunoprecipitated with either clone G99-549 (lanes 1 & 3) or clone G3-245 (lanes 2 & 4). G99-549 (ABIN967418) recognizes only underphosphorylated Rb whereas G3-245 (ABIN967405) recognizes all species of Rb (highly phosphorylated, phosphorylated and under phosphorylated). Lanes 1 & 2 - When P32-labeled IP extracts are run on a gel and detected via autoradiography, the radioactive label is greatly reduced in the G99-549 (lane 1) compared to the G3-245 (lane 2) immunoprecipitates. Lanes 3 & 4 - A western blot of the same gel probing for total Rb (Clone G3-245) shows that underphosphorylated Rb is present in the G99-549 immunoprecipitates (lane 3) whereas underphosphorylated, phosphorylated, and highly phosphorylated forms are present in the G3-245 immunoprecipitates (lane 4). pRb, underphosphorylated Rb. ppRb, phosphorylated and highly phosphorylated forms of Rb.