Cyclin A (CCN-A) antibody

Details for Product No. ABIN967425, Supplier: Log in to see
Antigen
Reactivity
Human
58
34
20
17
2
2
1
1
1
Host
Mouse
36
30
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
57
29
25
19
17
14
10
6
3
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Brand BD Pharmingen™
Immunogen Human Cyclin A Recombinant Protein
Clone BF683
Isotype IgE
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin A
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. Cyclin A may also form a complex with the adenovirus oncoprotein E1A which has DNA binding activity. Human cyclin A has been reported to migrate between 54-60 kDa by SDS-PAGE and clone BF683 reportedly does not cross-react with mouse, rat or mink cyclin A.
Molecular Weight 54-60 kDa
Research Area Cardiovascular, Cell Cycle
Comment

Related Products: ABIN968586, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Cyclin A (CCN-A) antibody (ABIN967425) Western blot analysis of cyclin A. K-562 cell lysates (Human bone marrow myelogenous ...
Background publications Gong, Bhatia, Traganos, Darzynkiewicz: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995 (PubMed).

Gong, Traganos, Darzynkiewicz: "Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1." in: Experimental cell research, Vol. 220, Issue 1, pp. 226-31, 1995 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Cao, Faha, Dembski, Tsai, Harlow, Dyson: "Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F." in: Nature, Vol. 355, Issue 6356, pp. 176-9, 1992 (PubMed).

Faha, Ewen, Tsai, Livingston, Harlow: "Interaction between human cyclin A and adenovirus E1A-associated p107 protein." in: Science (New York, N.Y.), Vol. 255, Issue 5040, pp. 87-90, 1992 (PubMed).