Cyclin D3 (CCND3) (full length) antibody

Details for Product No. ABIN967432
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Synonyms zgc:103502, CCND3, 9230106B05Rik, AA682053, AL024085, AW146355, C78795
full length
(37), (15), (12), (11), (10), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(178), (65), (59), (3), (2), (2), (2), (2)
(117), (61)
Clonality (Clone)
Monoclonal ()
(9), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
(120), (54), (48), (37), (29), (26), (21), (20), (16), (10), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
Quantity 0.1 mg
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Catalog No. ABIN967432
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Immunogen Recombinant full-length human cyclin D3
Clone G107-565
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin D3
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27Kip1. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large TAg), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. It is thought that D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of D-type cyclins are as follows: cyclin D1 (36 kDa), cyclin D2 (35 kDa) and cyclin D3 [31 and 34 kDa (doublet)]. G107-565 recognizes human cyclin D3. It does not cross-react with human cyclins D1 or D2.
Molecular Weight 31/34 kDa doublet
Research Area Cancer, Cell Cycle
Application Notes D-type cyclins are differentially expressed in distinct cell types. Cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls. WI-38 human diploid fibroblasts (ATCC CCL-75) and U-118 (ATCC HTB-15) are suggested as positive controls for detecting cyclin D1 and D3. Primary human peripheral blood T lymphocytes stimulated with phytohemagglutinin (PHA) are also good positive controls for detecting cyclin D3. The conjugated format of the clone is recommended for use in staining and flow cytometry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin D3 (CCND3) (full length) antibody Western blot analysis of cyclin D3. Lysate from K-562 human leukemia cells was probed with anti-cyclin D3 (clone G107-565, ABIN967432) at concentrations of 1.0, (lane 1), 0.1 (lane 2), and 0.01 µg/ml (lane 3). Cyclin D3 is identified as a band of ~34 kDa.
anti-Cyclin D3 (CCND3) (full length) antibody (2) anti-Cyclin D3 (CCND3) (full length) antibody (Image 2)
General Darzynkiewicz, Gong, Juan et al.: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).

Rao, Kohtz: "Positive and negative regulation of D-type cyclin expression in skeletal myoblasts by basic fibroblast growth factor and transforming growth factor beta. A role for cyclin D1 in control of myoblast differentiation." in: The Journal of biological chemistry, Vol. 270, Issue 8, pp. 4093-100, 1995 (PubMed).

Shapiro, Edwards, Kobzik et al.: "Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines." in: Cancer research, Vol. 55, Issue 3, pp. 505-9, 1995 (PubMed).

Gong, Bhatia, Traganos et al.: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995 (PubMed).

Gong, Traganos, Darzynkiewicz: "Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase." in: Cell proliferation, Vol. 28, Issue 6, pp. 337-46, 1995 (PubMed).

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