Cyclin D3 (CCND3) (full length) antibody
|Synonyms||CYCE2, C78795, AA682053, AL024085, AW146355, 9230106B05Rik, MGC93643, MGC108760, zgc:103502, CCND3, MGC128700, LOC780426|
Alternatives Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
|7 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||Cyclin D3|
|Immunogen||Recombinant full-length human cyclin D3|
|Description||Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27Kip1. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large TAg), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. It is thought that D-type cyclins are involved in regulating the passage of mammalian cells through G1. The reduced molecular weights of D-type cyclins are as follows: cyclin D1 (36 kDa), cyclin D2 (35 kDa) and cyclin D3 [31 and 34 kDa (doublet)]. G107-565 recognizes human cyclin D3. It does not cross-react with human cyclins D1 or D2.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||31/34 kDa doublet|
|Application Notes||D-type cyclins are differentially expressed in distinct cell types. Cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls. WI-38 human diploid fibroblasts (ATCC CCL-75) and U-118 (ATCC HTB-15) are suggested as positive controls for detecting cyclin D1 and D3. Primary human peripheral blood T lymphocytes stimulated with phytohemagglutinin (PHA) are also good positive controls for detecting cyclin D3. The conjugated format of the clone is recommended for use in staining and flow cytometry.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Cancer, Cell Cycle|
|Restrictions||For Research Use only|
Gong, Traganos, Darzynkiewicz: "Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase." in: Cell proliferation, Vol. 28, Issue 6, pp. 337-46, 1995 (PubMed).
Gong, Bhatia, Traganos et al.: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 9, Issue 5, pp. 893-9, 1995 (PubMed).
Shapiro, Edwards, Kobzik et al.: "Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines." in: Cancer research, Vol. 55, Issue 3, pp. 505-9, 1995 (PubMed).
Rao, Kohtz: "Positive and negative regulation of D-type cyclin expression in skeletal myoblasts by basic fibroblast growth factor and transforming growth factor beta. A role for cyclin D1 in control of myoblast differentiation." in: The Journal of biological chemistry, Vol. 270, Issue 8, pp. 4093-100, 1995 (PubMed).
Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).
Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).
Darzynkiewicz, Gong, Juan et al.: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997 (PubMed).