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Transcription Factor 3 (TCF3) antibody

Details for Product No. ABIN967433
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Antigen
Synonyms Pan2, Tcfe2a, TCF-3, TCF3
Reactivity
Human
(3), (3), (1)
Host
Mouse
(3), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)
(4), (2), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN967433
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Immunogen Recombinant Human E12
Clone G98-271
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name E2A (E12/E47)
Background B lymphoid cells go through many intermediate steps before becoming plasma ells whichproduce and secrete immunoglobulins (Ig). The earliest stage of B-cell differentiate is represented by pro-B lymphocytes that contain both the Ig heavy- and light-chain genes in the transcriptionally inactive germ line cnfiguration. As ro-B lymphocytes differentiate into pro-B cells, Ig heavy-chain genes rearrange and get transcribed and translated. However, the Ig lightchain genes are not transcribed before the pre-B cells differentiate into mature B-lymphocytes. The developmental regulation of the Ig gene expression is dependent on various sequences in the Ig enhancer region. One class of such regulatory sequence elements comprises the so-called E-boxes which share the NNCANNTGNN consensus sequence. The E2 boxes are particularly interesting because they are present in muscle and pancreas-specific enhancers. A family of proteins binds to the E2 box. These proteins share a common amino acid sequence motif that is proposed to form two amphipathic helices interrupted by a loop, designated the helix-loop-helix (HLH) motif. The HLH motif mediates homo- as well as heterodimerization with other HLH proteins. Most HLH proteins possess a basic region located N terminal of the HLH region which is responsible for DNA binding. Two E2 box binding proteins have been described (E12 and E47) that arise as alternatively spliced form of the E2A gene. E12 and E47 are identical except in the HLH region. These two proteins have been directly implicated in the regulation of B-cell, muscle and pancreas-specific gene expression. The E2-2 gene product is closely related to the E2A gene products but is encoded by a separate gene. E47 migrates at a reduced molecular weight of ~75 kDa. G98-271 reacts specifically with human E12 and E47 protein, alternatively spliced products of the E2A gene. It does not cross-react with the E2-2 protein. The specificity of the antibody was tested by immunoprecipitation and electrophoretic mobility band shift assays (EMSA) using recombinant protein (E2-2, E12, and E4 7). The HLH motif as well as flanking regions of recombinant human E12 potein was used as the immunogen.
Molecular Weight 75 kDa (E12, E47)
Application Notes Applications include immunoprecipitation, western blot analysis (1-2 µg/ml) and electrophoretic mobility band shift assays (EMSA).
Positive controls: E2A proteins (E12 and E47) have been detected in cells derived from both B-cell and T-cell lineages including the pre B-cell line Nalm-6, the mature B-cell line Namalwa (ATCC CRL-1432), the T-cell line Jurkat (ATCC TIB-152). E2A has also been detected in HeLa cervical carcinoma cells (ATCC CCL-2).
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Transcription Factor 3 (TCF3) antibody Western blot analysis of E12/E47. Lysate from Jurkat cells was probed with E12/E47 antibody (clone G98-271) at concentrations of 2.0, 1.0, and 0.5 myg/ml. The antibody identifies E12/R47 as a 75 kDa band.
anti-Transcription Factor 3 (TCF3) antibody (2) anti-Transcription Factor 3 (TCF3) antibody (Image 2)
General Sun, Baltimore: "An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers." in: Cell, Vol. 64, Issue 2, pp. 459-70, 1991 (PubMed).

Whelan, Cordle, Henderson et al.: "Identification of a pancreatic beta-cell insulin gene transcription factor that binds to and appears to activate cell-type-specific expression: its possible relationship to other cellular factors that bind to a common insulin gene sequence." in: Molecular and cellular biology, Vol. 10, Issue 4, pp. 1564-72, 1990 (PubMed).

Murre, McCaw, Baltimore: "A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins." in: Cell, Vol. 56, Issue 5, pp. 777-83, 1989 (PubMed).

Buskin, Hauschka: "Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene." in: Molecular and cellular biology, Vol. 9, Issue 6, pp. 2627-40, 1989 (PubMed).

Lenardo, Pierce, Baltimore: "Protein-binding sites in Ig gene enhancers determine transcriptional activity and inducibility." in: Science (New York, N.Y.), Vol. 236, Issue 4808, pp. 1573-7, 1987 (PubMed).

Bain, Gruenwald, Murre: "E2A and E2-2 are subunits of B-cell-specific E2-box DNA-binding proteins." in: Molecular and cellular biology, Vol. 13, Issue 6, pp. 3522-9, 1993 (PubMed).

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