Cyclin D2 (CCND2) antibody

Antigen: Cyclin D2 (CCND2)

Synonyms: KIAK0002, MGC102758, cD2, Vin1, Vin-1, C86853, AI256817, BF642806, 2600016F06Rik, ccnd2-A, CCND2, si:ch211-240j22.2, MGC142404, MGC132398, ccnd2, kiak0002, LOC100224096

Clonality: Monoclonal (G132-43)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967434

Additional Information

Alternative name: Cyclin D2

Immunogen: Recombinant Human Cyclin D2

Format: Liquid

Isotype: IgG2a

Clone: G132-43

Description: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating or inhibitory phosphorylation events, as well as by interactions with small proteins, e.g., p16 and p27, that bind to cyclins, cdks, and cyclin-cdk complexes. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, cabl, SV40 large T-Ag), tumor suppressor genes (retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are expressed mainly in G1 and play a role in regulating the passage of mammalian cells through the G1 phase of the cell cycle. The reduced molecular weights of D-type cyclins are as follows: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 (31 and 34 kDa [doublet]). G132-43 recognizes human cyclin D2. It does not cross-react with human cyclins D1 and D3. Recombinant, full-length human cyclin D2 produced in the Baculovirus expression system (BEVS) was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 35 kDa

Comments:

Related Products: ABIN967389, ABIN968553

Application Details

Application Notes: Applications include western blotting (1-2 µg/ml) and immunoprecipitation (1-2 µg/one million cells). D-type cyclins are differentially expressed in distinct cell types, therefore cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls for these applications. WI-38 human diploid fibroblasts (ATCC CCL 75) and U-118 MG human glioblastoma cells (ATCC HTB 15) are suggested as positive controls for detection of cyclin D1 and D3. Primary human peripheral blood T lymphocytes stimulated with phytohemagglutin (PHA) and Raji human Burkitt lymphoma cells (ATCC CCL 86) are suggested as positive controls for detecting cyclin D2.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).