Cyclin D2 (CCND2) antibody

Details for Product No. ABIN967434
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Antigen
Synonyms si:ch211-240j22.2, ccnd2, CCND2, kiak0002, zgc:162280, LOC100224096, KIAK0002, 2600016F06Rik, AI256817, BF642806, C86853, Vin-1, Vin1, cD2
Reactivity
Human
(113), (55), (41), (1), (1), (1)
Host
Mouse
(70), (47)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(92), (32), (32), (23), (15), (15), (10), (10), (9), (9), (7), (6), (5), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967434
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Immunogen Recombinant Human Cyclin D2
Clone G132-43
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin D2
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating or inhibitory phosphorylation events, as well as by interactions with small proteins, e.g., p16 and p27, that bind to cyclins, cdks, and cyclin-cdk complexes. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, cabl, SV40 large T-Ag), tumor suppressor genes (retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are expressed mainly in G1 and play a role in regulating the passage of mammalian cells through the G1 phase of the cell cycle. The reduced molecular weights of D-type cyclins are as follows: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 (31 and 34 kDa [doublet]). G132-43 recognizes human cyclin D2. It does not cross-react with human cyclins D1 and D3. Recombinant, full-length human cyclin D2 produced in the Baculovirus expression system (BEVS) was used as immunogen.
Molecular Weight 35 kDa
Research Area Cancer, Cell Cycle
Application Notes Applications include western blotting (1-2 µg/ml) and immunoprecipitation (1-2 µg/one million cells). D-type cyclins are differentially expressed in distinct cell types, therefore cell types which have been documented to express high levels of a given D-type cyclin are suggested as positive controls for these applications. WI-38 human diploid fibroblasts (ATCC CCL 75) and U-118 MG human glioblastoma cells (ATCC HTB 15) are suggested as positive controls for detection of cyclin D1 and D3. Primary human peripheral blood T lymphocytes stimulated with phytohemagglutin (PHA) and Raji human Burkitt lymphoma cells (ATCC CCL 86) are suggested as positive controls for detecting cyclin D2.
Comment

Related Products: ABIN967389, ABIN968553

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
General Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

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