Deleted in Colorectal Carcinoma (DCC) (Extracellular Domain) antibody

Antigen: Deleted in Colorectal Carcinoma (DCC)

Synonyms: CRC18, CRCR1, IGDCC1, Igdcc1, C030036D22Rik, DCC, zdcc, xdcc, XDCCa, dcca-A

Binding Site: Extracellular Domain

Clonality: Monoclonal (G92-13)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN967442

Additional Information

Alternative name: DCC

Immunogen: Recombinant Human DCC

Format: Liquid

Isotype: IgG1

Clone: G92-13

Description: One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kD glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family. DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas. The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. G92-13 recognizes human DCC. A truncated recombinant protein containing the extracellular domain of the human DCC was used as immunogen.
Synonyms: Deleted in Colon Cancer

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 175-190 kDa

Comments:

Related Products: ABIN967443

Application Details

Application Notes: IMR 32 human neuroblastoma cells (ATCC CCL 127) are suggested as a positive control. By western blot, DCC-specific antibodies typically identify protein species with molecular weights of ~175-190 kD. Doublets in this range have been reported in brain. Several smaller immunoreactive species, representing degradation products, cross-reactive species, or DCC forms arising from alternative splicing of DCC mRNA or in vivo processing of the DCC protein may also be identified. Another DCC clone (mAb G97-449, ABIN967443) has been published for immunohistochemical staining of paraffin-embedded tissue sections.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of human DCC protein in 293 human embryonic kidney cells stably transfected with an expression vector containing full length DCC cDNA. Lane 1, clone G97-449 (ABIN967443), which recognizes an epitope in the intracellular domain of DCC. Lane 2, clone G92-13 (ABIN967442), which recognizes an epitope in the extracellular domain of DCC. Lane 3, a mouse IgG1 isotype control.

anti-Deleted in Colorectal Carcinoma (DCC) (Extracellular Domain) antibody (Image 2)

Publications

Fearon, Cho, Nigro et al.: "Identification of a chromosome 18q gene that is altered in colorectal cancers." in: Science (New York, N.Y.), Vol. 247, Issue 4938, pp. 49-56, 1990 (PubMed).

Vogelstein, Fearon, Hamilton et al.: "Genetic alterations during colorectal-tumor development." in: The New England journal of medicine, Vol. 319, Issue 9, pp. 525-32, 1988 (PubMed).

Fearon, Hamilton, Vogelstein: "Clonal analysis of human colorectal tumors." in: Science (New York, N.Y.), Vol. 238, Issue 4824, pp. 193-7, 1987 (PubMed).

Reale, Hu, Zafar et al.: "Expression and alternative splicing of the deleted in colorectal cancer (DCC) gene in normal and malignant tissues." in: Cancer research, Vol. 54, Issue 16, pp. 4493-501, 1994 (PubMed).

Shibata, Reale, Lavin et al.: "The DCC protein and prognosis in colorectal cancer." in: The New England journal of medicine, Vol. 335, Issue 23, pp. 1727-32, 1996 (PubMed).