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p21 antibody

CDKN1A Reactivity: Human WB, IHC (p), IP, IHC (fro) Host: Mouse Monoclonal 6B6 unconjugated
Catalog No. ABIN967445
  • Target See all p21 (CDKN1A) Antibodies
    p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
    Reactivity
    • 250
    • 117
    • 94
    • 36
    • 14
    • 12
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 216
    • 54
    • 1
    Mouse
    Clonality
    • 209
    • 61
    • 1
    Monoclonal
    Conjugate
    • 112
    • 20
    • 18
    • 18
    • 13
    • 11
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This p21 antibody is un-conjugated
    Application
    • 180
    • 109
    • 67
    • 66
    • 66
    • 66
    • 62
    • 43
    • 41
    • 33
    • 19
    • 16
    • 15
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Brand
    BD Pharmingen™
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human p21
    Clone
    6B6
    Isotype
    IgG1
    Top Product
    Discover our top product CDKN1A Primary Antibody
  • Application Notes
    Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of paraformaldehyde-fixed cultured cells (0.5-2.5 µg/ml) and acetone-fixed, frozen and formalin-fixed paraffin-embedded (10 µg/ml) tissue sections. MCF7 human breast carcinoma cells (ATCC HTB-22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL-75) treated with doxorubin (Adriamycin) can also be used as a positive control.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Clarke, Lotz, Chao, Mercurio: "Activation of the p21 pathway of growth arrest and apoptosis by the beta 4 integrin cytoplasmic domain." in: The Journal of biological chemistry, Vol. 270, Issue 39, pp. 22673-6, (1995) (PubMed).

    Halevy, Novitch, Spicer, Skapek, Rhee, Hannon, Beach, Lassar: "Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD." in: Science (New York, N.Y.), Vol. 267, Issue 5200, pp. 1018-21, (1995) (PubMed).

    el-Deiry, Harper, OConnor, Velculescu, Canman, Jackman, Pietenpol, Burrell, Hill, Wang: "WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis." in: Cancer research, Vol. 54, Issue 5, pp. 1169-74, (1994) (PubMed).

    Hunter: "Braking the cycle." in: Cell, Vol. 75, Issue 5, pp. 839-41, (1994) (PubMed).

    Noda, Ning, Venable, Pereira-Smith, Smith: "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen." in: Experimental cell research, Vol. 211, Issue 1, pp. 90-8, (1994) (PubMed).

    Xiong, Hannon, Zhang, Casso, Kobayashi, Beach: "p21 is a universal inhibitor of cyclin kinases." in: Nature, Vol. 366, Issue 6456, pp. 701-4, (1994) (PubMed).

    el-Deiry, Tokino, Velculescu, Levy, Parsons, Trent, Lin, Mercer, Kinzler, Vogelstein: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, (1993) (PubMed).

  • Target
    p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
    Alternative Name
    p21 (CDKN1A Products)
    Synonyms
    CCND1 antibody, CDKN1A antibody, CAP20 antibody, CDKN1 antibody, CIP1 antibody, MDA-6 antibody, P21 antibody, SDI1 antibody, WAF1 antibody, p21CIP1 antibody, CDKI antibody, Cdkn1 antibody, Waf1 antibody, mda6 antibody, p21Cip1 antibody, p21WAF antibody, Cip1 antibody, UV96 antibody, p21 antibody, cip1 antibody, p16Xic2 antibody, sdi1 antibody, waf1 antibody, cap20 antibody, cdkn1 antibody, mda-6 antibody, p21cip1 antibody, si:ch1073-48m5.2 antibody, KRAS2 antibody, p21ras antibody, cyclin D1 antibody, cyclin dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A (P21) antibody, cyclin-dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A L homeolog antibody, cyclin-dependent kinase inhibitor 1 antibody, KRAS proto-oncogene, GTPase antibody, CCND1 antibody, CDKN1A antibody, Cdkn1a antibody, cdkn1a.L antibody, cki1 antibody, cdkn1a antibody, KRAS antibody
    Background
    P21 belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21 and p53-regulated inhibitor of Cdks (Pic1). p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. When introduced into proliferating foreskin fibroblasts, p21 inhibits DNA synthesis and cell cycle arrest. p53 can activate p21 gene expression via a p53 binding site identified in the promoter of the p21 gene.
    Molecular Weight
    21 kDa
    Pathways
    p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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