GTPase Kras antibody
|Synonyms||NS, NS3, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, ras, p21B, K-ras, Kras2, Ki-ras, Kras-2, MGC7141, AI929937|
Alternatives Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunoprecipitation (IP)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Human p21 fusion protein|
|Description||The p21 protein belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21, and p53-regulated inhibitor of Cdks (Pic1). The 2.1 kb cDNA coding for p21 was first cloned from a library made from senescent normal human foreskin fibroblasts. When introduced into proliferating foreskin fibroblasts it causes inhibition of DNA synthesis and cell cycle arrest. The p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. The resultant protein has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS/PAGE. p21 is believed to function by inhibiting the kinase activity of the cyclin-cdk complexes to which it binds. p53 has been shown to cause the induction of p21 gene, presumably by recognizing a p53 binding site identified in the promoter of the p21 gene. Clone 2G12 recognizes human p21. A full-length human p21 fusion protein was expressed in bacteria and used as immunogen. Mice were immunized and hybridomas were selected for reactivity against p21 by ELISA and western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||21 kDa|
Related Products: ABIN967389, ABIN968585
|Application Notes||Applications include immunoprecipitation. (1-2 µg/one million cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of antigen unmasked, paraffin-embedded tissue sections (5-20 µg/ml). MCF-7 human breast carcinoma cells (ATCC HTB 22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL 75) treated with doxorubin (Adriamycin) can also be used as a positive control.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Cell Cycle|
|Restrictions||For Research Use only|
el-Deiry, Harper, OConnor et al.: "WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis." in: Cancer research, Vol. 54, Issue 5, pp. 1169-74, 1994 (PubMed).
Noda, Ning, Venable et al.: "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen." in: Experimental cell research, Vol. 211, Issue 1, pp. 90-8, 1994 (PubMed).
el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).
Hunter: "Braking the cycle." in: Cell, Vol. 75, Issue 5, pp. 839-41, 1994 (PubMed).
Xiong, Hannon, Zhang et al.: "p21 is a universal inhibitor of cyclin kinases." in: Nature, Vol. 366, Issue 6456, pp. 701-4, 1994 (PubMed).
|Hosts||Rabbit (47), Mouse (8), Goat (1), Rat (1)|
|Reactivities||Human (50), Rat (Rattus) (33), Mouse (Murine) (27), Cow (Bovine) (5), Pig (Porcine) (3), Rabbit (3), Mammalian (2), Cat (Feline) (1), Chicken (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (36), ELISA (27), Immunofluorescence (IF) (24), Immunohistochemistry (IHC) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Biotin (3), FITC (2), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (3), Internal Region (1), pSer145 (1), pThr145 (1)|