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K-RAS antibody (GTPase Kras)

Details for Product anti-GTPase Kras Antibody No. ABIN967448, Supplier: Log in to see
Antigen
  • Kras2
  • c-Ki-ras
  • p21
  • C-K-RAS
  • CFC2
  • K-RAS2A
  • K-RAS2B
  • K-RAS4A
  • K-RAS4B
  • KI-RAS
  • KRAS1
  • KRAS2
  • NS
  • NS3
  • RASK2
  • SIIR
  • pp21
  • 0610011M09Rik
  • BC011290
  • P21
  • AI929937
  • K-ras
  • Ki-ras
  • Kras-2
  • p21B
  • ras
  • wu:fa04e08
  • wu:fc14b12
  • wu:fc23g10
  • wu:fj89d12
  • zgc:85725
Reactivity
Human
220
58
47
37
6
5
4
4
4
3
2
1
Host
Mouse
174
89
Clonality (Clone)
Monoclonal ()
Conjugate
This K-RAS antibody is un-conjugated
14
14
13
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
181
139
72
62
44
27
24
23
22
4
3
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Human p21 fusion protein
Clone 2G12
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p21
Background The p21 protein belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21, and p53-regulated inhibitor of Cdks (Pic1). The 2.1 kb cDNA coding for p21 was first cloned from a library made from senescent normal human foreskin fibroblasts. When introduced into proliferating foreskin fibroblasts it causes inhibition of DNA synthesis and cell cycle arrest. The p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. The resultant protein has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS/PAGE. p21 is believed to function by inhibiting the kinase activity of the cyclin-cdk complexes to which it binds. p53 has been shown to cause the induction of p21 gene, presumably by recognizing a p53 binding site identified in the promoter of the p21 gene. Clone 2G12 recognizes human p21. A full-length human p21 fusion protein was expressed in bacteria and used as immunogen. Mice were immunized and hybridomas were selected for reactivity against p21 by ELISA and western blot analysis.
Molecular Weight 21 kDa
Research Area Cell Cycle
Pathways
Application Notes Applications include immunoprecipitation. (1-2 µg/one million cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of antigen unmasked, paraffin-embedded tissue sections (5-20 µg/ml). MCF-7 human breast carcinoma cells (ATCC HTB 22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL 75) treated with doxorubin (Adriamycin) can also be used as a positive control.
Comment

Related Products: ABIN967389, ABIN968585

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-K-RAS antibody (GTPase Kras) (ABIN967448) Western blot analysis of p21 expression. Lysates from MCF-7 cells (50 µg/lane) were p...
 image for anti-K-RAS antibody (GTPase Kras) (ABIN967448) anti-GTPase Kras antibody (Image 2)
Western Blotting (WB) image for anti-K-RAS antibody (GTPase Kras) (ABIN967448) anti-GTPase Kras antibody (Image 3)
Product cited in: Xiong, Hannon, Zhang et al.: "p21 is a universal inhibitor of cyclin kinases." in: Nature, Vol. 366, Issue 6456, pp. 701-4, 1994 (PubMed).

Hunter: "Braking the cycle." in: Cell, Vol. 75, Issue 5, pp. 839-41, 1994 (PubMed).

el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).

Noda, Ning, Venable et al.: "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen." in: Experimental cell research, Vol. 211, Issue 1, pp. 90-8, 1994 (PubMed).

el-Deiry, Harper, OConnor et al.: "WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis." in: Cancer research, Vol. 54, Issue 5, pp. 1169-74, 1994 (PubMed).