GTPase Kras antibody

Details for Product No. ABIN967448
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Antigen
Synonyms
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu: ... show more
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu:fa04e08, wu:fc14b12, wu:fc23g10, wu:fj89d12, zgc:85725 show less
Reactivity
Human
(98), (40), (40), (13), (2), (1), (1), (1), (1)
Host
Mouse
(83), (22)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections), Immunoprecipitation (IP)
(81), (47), (24), (20), (12), (10), (9), (4), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967448
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Immunogen Human p21 fusion protein
Clone 2G12
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p21
Background The p21 protein belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21, and p53-regulated inhibitor of Cdks (Pic1). The 2.1 kb cDNA coding for p21 was first cloned from a library made from senescent normal human foreskin fibroblasts. When introduced into proliferating foreskin fibroblasts it causes inhibition of DNA synthesis and cell cycle arrest. The p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. The resultant protein has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS/PAGE. p21 is believed to function by inhibiting the kinase activity of the cyclin-cdk complexes to which it binds. p53 has been shown to cause the induction of p21 gene, presumably by recognizing a p53 binding site identified in the promoter of the p21 gene. Clone 2G12 recognizes human p21. A full-length human p21 fusion protein was expressed in bacteria and used as immunogen. Mice were immunized and hybridomas were selected for reactivity against p21 by ELISA and western blot analysis.
Molecular Weight 21 kDa
Research Area Cell Cycle
Application Notes Applications include immunoprecipitation. (1-2 µg/one million cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of antigen unmasked, paraffin-embedded tissue sections (5-20 µg/ml). MCF-7 human breast carcinoma cells (ATCC HTB 22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL 75) treated with doxorubin (Adriamycin) can also be used as a positive control.
Comment

Related Products: ABIN967389, ABIN968585

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-GTPase Kras antibody Western blot analysis of p21 expression. Lysates from MCF-7 cells (50 µg/lane) were probed with anti- human p21 (clone 2G12, ABIN967448) followed by alkaline phosphatase labeled anti-mouse second-step polyclonal antibodies. The antibody recognizes p21 as a 21 kDa protein.
anti-GTPase Kras antibody (2) anti-GTPase Kras antibody (Image 2)
General el-Deiry, Harper, OConnor et al.: "WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis." in: Cancer research, Vol. 54, Issue 5, pp. 1169-74, 1994 (PubMed).

Noda, Ning, Venable et al.: "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen." in: Experimental cell research, Vol. 211, Issue 1, pp. 90-8, 1994 (PubMed).

el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).

Hunter: "Braking the cycle." in: Cell, Vol. 75, Issue 5, pp. 839-41, 1994 (PubMed).

Xiong, Hannon, Zhang et al.: "p21 is a universal inhibitor of cyclin kinases." in: Nature, Vol. 366, Issue 6456, pp. 701-4, 1994 (PubMed).

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