anti-IL-10 antibody (Interleukin 10)

Details for Product anti-IL10 Antibody No. ABIN967464
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Antigen
Synonyms CSIF, GVHDS, IL-10, IL10A, TGIF, Il-10, IL10X
Reactivity
Human
(342), (199), (74), (23), (11), (6), (6), (6), (2), (1), (1)
Host
Rat
(228), (211), (134), (8), (6), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
This IL-10 antibody is un-conjugated
(102), (30), (24), (11), (10), (9), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA (Capture), Flow Cytometry (FACS), Western Blotting (WB)
(384), (378), (136), (82), (79), (46), (45), (32), (30), (24), (19), (13), (12), (11), (9), (8), (8), (7), (6), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mg
Options
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Immunogen COS-expressed Recombinant Human IL-10
Clone JES3-9D7
Isotype IgG1
Cross-Reactivity Virus
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IL-10 (IL10 Antibody Abstract)
Background The JES3-9D7 antibody reacts with human IL-10 and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed in COS cells. This antibody is routinely tested by intracellular staining.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Application Notes Blocking Control for Intracellular Staining:
The purified JES3-9D7 antibody can be used as a blocking control to demonstrate specificity of IL-10 staining by PE-JES3-9D7 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled JES3-9D7 antibody (ABIN967464) for 20 minutes at 4°C, prior to staining with PE-JES3-9D7 antibody (e.g., 0.1 - 0.5 µg mAb/1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

Neutralization:
The NA/LE format of the JES3-9D7 antibody is useful for neutralization of human or viral IL-10 bioactivity. A suitable NA/LE™ rat IgG1 isotype control to match the NA/LE™ JES3-9D7 antibody is the R3-34 antibody.

3. WB: The JES3-9D7 antibody (e.g., ABIN967464) has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Interleukin 10 (IL10) antibody Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated f...
anti-Interleukin 10 (IL10) antibody (2) Recombinant cytokine blocking
anti-Interleukin 10 (IL10) antibody (3) Unlabeled antibody
Product cited in: Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008 (PubMed).

General Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Burdin, Péronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed).

Kesslak, Yuan, Neeper et al.: "Vulnerability of the hippocampus to kainate excitotoxicity in the aged, mature and young adult rat." in: Neuroscience letters, Vol. 188, Issue 2, pp. 117-20, 1995 (PubMed).

Just, Abrams, Louie et al.: "Influence of host genotype on progression to acquired immunodeficiency syndrome among children infected with human immunodeficiency virus type 1." in: The Journal of pediatrics, Vol. 127, Issue 4, pp. 544-9, 1995 (PubMed).

Gotlieb, Abrams, Watson et al.: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).

Validation Images
Catalog No. ABIN967464
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