Images for product: anti-Interleukin 10 (IL10) antibody
Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hours with LPS (1.0 µg/ml final concentration) in the presence of GolgiStop™ (2 µM final concentration). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2), fixed, permeabilized, and subsequently stained with 0.25 µg of PE-rat anti-human IL-10 antibody (PE-JES3-9D7) following Pharmingen's staining protocol (first panel). The data reflect gating on monocytes, based on forward and side light scatter. To demonstrate specificity of staining, the binding of PE-JES3-9D7 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 (0.5 µg, middle panel), and by preincubation of the fixed/permeabilized cells with the unlabeled JES3-9D7 antibody (5.0 µg, ABIN967464, second panel) prior to staining with the PE-JES3-9D7 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody (second panel) blocking specificity controls.