Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) antibody

Antigen: Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB)

Synonyms: CD131, IL3RB, IL5RB, CDw131, Csf2rb1, CSF2RB, il-3rbc

Clonality: Monoclonal (3D7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN967466

Additional Information

Alternative name: CDw131

Immunogen: Human IL-3 transfected cells

Format: Liquid

Isotype: IgG1, kappa

Clone: 3D7

Description: The 3D7 antibody reacts with CDw131 the 120 kD common beta chain (betac) which is shared with the receptor complexes for human granulocyte-macrophage colony stimulating factor (GM-CSFR), interleukin-3 (IL-3R) and interleukin-5 (IL-5R). Together with the alpha subunit of either the IL-3R (IL-3Ralpha), IL-5R (IL-5Ralpha), or GM- CSFR (GM-CSFRalpha), the common beta chain forms high-affinity, signaling receptors for human IL-3, IL-5 and GM-CSF, respectively. Cell surface betac are expressed by a variety of different cell types including hematopoietic progenitor cells derived from pluripotent stem cells, monocytes, neutrophils, eosinophils, basophils, endothelial cells, fibroblasts, and Langerhans cells. The immunogen used to generate this hybridoma was cells co-transfected with expression vectors which contained cDNA for the human IL-3 alpha and beta chains.
Synonyms: Cytokine receptor common beta chain

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Application Notes: Immunofluorescent Staining and Flow Cytometric Analysis:
The various formats of the 3D7 antibody are useful for the immunofluorescent staining and flow cytometric analysis of cells for betac expression. The purified format (ABIN967466) of this antibody can be used in conjunction with biotinylated goat anti-mouse Ig with no cross reactivity to human Ig and streptavidin-phycoerythrin in a three-layer staining procedure to amplify immunofluorescent signals for the flow cytometric analysis of cytokine receptor-expressing cells.

Immunoprecipitation: This purified 3D7 antibody (ABIN967466) can be used to immunoprecipitate betac receptors. Please note that this application is not routinely tested.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Publications

Woodcock, Zacharakis, Plaetinck et al.: "Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF." in: The EMBO journal, Vol. 13, Issue 21, pp. 5176-85, 1994 (PubMed).

Product: Zola: "Detection of cytokine receptors by flow cytometry." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.21, 2008 (PubMed).