Images for product: anti-Interferon gamma (IFNG) antibody
Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 6 hr with PMA (50 ng/ml, Sigma) and calcium ionophore A23187 (500 ng/ml, Sigma) in the presence of 2 µM BD GolgiStop™. The PBMC were stained with PE-Cy5-anti-CD3 (PE-Cy5 UCHT1), fixed, permeabilized, and subsequently stained with 0.125 µg of PE-mouse anti-human IFN-gamma antibody (PE-4S.B3) by using Pharmingen's staining protocol (first panel). The binding of PE-4S.B3 was blocked by preincubation of cells with unlabeled 4S.B3 antibody (5 µg, second panel). The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls.