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IFNG antibody (Interferon gamma)

Details for Product anti-IFNG Antibody No. ABIN967468, Supplier: Login to see
Antigen
  • IFNG2
  • IFN-g
  • Ifg
  • IFN-G
  • IFN-gamma
  • IFNG
  • IFG
  • IFI
Alternatives
anti-Human IFNG antibody for Enzyme Immunoassay
Reactivity
Human
635
272
150
57
36
25
21
20
13
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11
9
9
9
9
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6
6
6
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2
1
1
1
Host
Mouse
545
396
173
35
8
7
4
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IFNG antibody is un-conjugated
159
115
63
46
36
12
10
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9
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3
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3
3
3
2
2
2
2
2
2
1
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1
Application
ELISA (Detection), Flow Cytometry (FACS), Western Blotting (WB)
651
571
363
125
125
117
75
61
48
41
38
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32
31
17
15
9
9
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4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Partially purified human IFN-gamma from supernatants of human PBMC stimulated with Staphylococcus aureus
Clone 4S-B3
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IFN-gamma (IFNG Antibody Abstract)
Background The 4S.B3 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate this hybridoma was partially purified human IFN-gamma obtained from supernatants of human PBMC stimulated with Staphylococcus aureus. This is a neutralizing antibody.
Research Area Cytokines
Pathways Interferon-gamma Pathway
Application Notes Blocking Control for Intracellular Staining:
The purified 4S.B3 antibody (ABIN967468) can be used as a blocking control to demonstrate specificity of IFN-gamma staining by directly conjugated clone 4S.B3. To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1-10 µg of purified 4S.B3 antibody (ABIN967468) for 20 minutes at 4°C, prior to staining with the directly conjugated antibody (e.g., 0.1 -0.5 µg mAb/1 million cells) (right panel). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

ELISA Detection:
The biotinylated 4S.B3 antibody is useful as a detection antibody in a sandwich ELISA for measuring human IFN-gamma protein levels. Biotinylated 4S.B3 antibody can be paired with the purified NIB42 antibody as the capture antibody, with recombinant human IFN-gamma as the standard. For testing human IFN-gamma complex in biological fluids like serum or plasma, our human IFN-gamma specific OptEIA™ sandwich ELISA set and OptEIA™ sandwich ELISA kit are recommended.

Western Blot:
The 4S.B3 antibody has been found useful for Western blotting.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
 image for anti-IFNG antibody (Interferon gamma) (ABIN967468) Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells (PBMC)...
 image for anti-IFNG antibody (Interferon gamma) (ABIN967468) The binding of PE-4S.B3 was blocked by preincubation of cells with unlabeled 4S.B3 an...
Background publications Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Meager, Parti, Barwick et al.: "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." in: Journal of interferon research, Vol. 4, Issue 4, pp. 619-25, 1985 (PubMed).