Glutathione S Transferase (GST) antibody

Details for Product No. ABIN967470
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Antigen
Synonyms GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1, GSTS, GSTS1-1, PGD2, PGDS, H-PGDS, Ptgds2, 1500002K10Rik, Gst
Reactivity
Mouse (Murine)
(108), (73), (25), (14), (5), (3), (3), (3), (2), (2), (1), (1), (1)
Host
Mouse
(131), (87), (59), (3), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(15), (15), (13), (8), (6), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(188), (142), (56), (46), (24), (19), (13), (9), (7), (6), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN967470
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Immunogen Recombinant GST-tagged protein
Clone G172-1138
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Glutathione S-transferase
Background Glutathione S-Transferase (GST) from Schistosoma japonicum is a 26 kD protein that has become a popular fusion partner for high expression of recombinant proteins. GST tags allow recombinant fusion proteins to be purified in a single nondenaturing affinity step on glutathione-coupled beads. The high affinity of the GST-glutathione interaction results in simple near-homogeneous purifications. Clone G172-1138 recognizes GST. A recombinant GST-tagged protein was used as immunogen.
Synonyms: GST
Application Notes Applications include western blot analysis (1-2 µg/ml antibody). The antibody is useful in screening for recombinant GST-tagged proteins produced in bacteria or in insect cells infrected with recombinant baculovirus.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
General Davies, Jowett, Jones: "Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins." in: Bio/technology (Nature Publishing Company), Vol. 11, Issue 8, pp. 933-6, 1993 (PubMed).

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