Neural Cell Adhesion Molecule 1 (NCAM1) antibody

Antigen: Neural Cell Adhesion Molecule 1 (NCAM1)

Synonyms: CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761

Clonality: Monoclonal (12F11)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN967496

Additional Information

Alternative name: CD56 (N-CAM)

Immunogen: BALB/c mouse thymocytes

Cross-Reactivity: Chicken, Human, Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 12F11

Description: CD56 or N-CAM (Neural cell adhesion molecule) is a member of a family of adhesion molecules including L1, contactin, TAG-1 and others, which mediate neuronal attachment, process extension and cell-cell interaction(s) in the central nervous system (CNS). N-CAM has been shown to play a role during the development and maintenence of synaptic connectivity of the hippocampus, as well as other organs within the CNS. In addition to facilitating cell adhesive properties, N-CAM may play a role signal transduction pathways. N-CAM can participate in regulation of astrocyte proliferation via a mechanism which involves the mitogen-activated protein kinase (MAPK) pathway. N-CAM associates directly with L1 to form a complex which participates in signal pathways promoting neurite outgrowth. N-CAM exists in several isoforms which result from alternative splicing of mRNA. These isoforms contain posttranslational modifications such as addition of polysialic residues and carbohydrate epitopes. Embryonic N-CAM (~200-230 kD) is highly sialylated, whereas adult N-CAM isoforms (~120, 140 and 180 kD) are less so, yet are more adhesive. These modifications, along with the diversity of local distribution of the isoforms, may contribute to distinct roles for these forms. Adult N-CAM isoforms of 120, 140 and 180 kDa are observed by SDS-PAGE.
Clone 12F11 recognizes an intracellular epitope of N-CAM and has been shown to react with human, rat, mouse, and chick N-CAM. The antibody specifically recognizes the 140 and 180 kDa isoforms of N-CAM, but does not recognize the 120 kDa isoform. Higher molecular weight (MW) isoforms may also be observed, particularly in samples from embryonic brain tissue.
Synonyms: N-CAM

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Application Notes: The MW of N-CAM isoforms may vary due to the degree of posttranslational modification of the proteins, thus the staining pattern of 12F11 may appear as a wide smear”in some samples. Other application not routinely tested includes immunohistochemical staining of paraformaldehyde-fixed, frozen tissue sections of human, mouse and rat origin. Please refer to methods described by Miller et al., 1993 for details regarding the use of 12F11 in this application.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of N-CAM. Brain tissue extracts from embryonic or adult BALB/c mice were separated by SDS-PAGE and probed with anti-N-CAM (ABIN967496). In embryonic extracts, highly sialylated isoforms of N-CAM, ≥200 kDa, are observed (lane 1), whereas in extracts from adult mice additional N-CAM isoforms, which are less sialylated, are also observed ≤180 kDa (lane 2).

Publications

Product: Chung, Lagenaur, Yan et al.: "Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb." in: The Journal of comparative neurology, Vol. 314, Issue 2, pp. 290-305, 1992 (PubMed).

Cunningham, Hemperly, Murray et al.: "Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing." in: Science (New York, N.Y.), Vol. 236, Issue 4803, pp. 799-806, 1987 (PubMed).

Santoni, Barthels, Barbas et al.: "Analysis of cDNA clones that code for the transmembrane forms of the mouse neural cell adhesion molecule (NCAM) and are generated by alternative RNA splicing." in: Nucleic acids research, Vol. 15, Issue 21, pp. 8621-41, 1988 (PubMed).

Miller, Chung, Lagenaur et al.: "Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus." in: The Journal of comparative neurology, Vol. 327, Issue 3, pp. 341-9, 1993 (PubMed).

Krushel, Tai, Cunningham et al.: "Neural cell adhesion molecule (N-CAM) domains and intracellular signaling pathways involved in the inhibition of astrocyte proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2592-6, 1998 (PubMed).

Heiland, Griffith, Lange et al.: "Tyrosine and serine phosphorylation of the neural cell adhesion molecule L1 is implicated in its oligomannosidic glycan dependent association with NCAM and neurite outgrowth." in: European journal of cell biology, Vol. 75, Issue 2, pp. 97-106, 1998 (PubMed).