Cyclin-Dependent Kinase 7 (CDK7) antibody

Antigen: Cyclin-Dependent Kinase 7 (CDK7)

Synonyms: CAK, p34, p37, CAK1, MO15, STK1, CDKN7, p39MO15, Crk4, Cdkn7, AI323415, AI528512, C230069N13, CDK7, DmCDK7, DmMO15, cdk-7, dCdk7, p40[MO15], DmelCG3319, CG3319, MGC85821, zgc:85821, MGC140417, cak1, stk1, cdkn7, p39mo15, p40MO15

Clonality: Monoclonal (MO-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN967506

Additional Information

Alternative name: Cdk7

Immunogen: Recombinant Human Cdk7

Format: Liquid

Isotype: IgG2b

Clone: MO-1

Description: Cyclins and cyclin-dependent kinases (Cdks) are subunits of cell cycle dependent protein kinases that regulate key events during the cell cycle. For orderly progression to take place, Cdks must become fully active only at appropriate times in the cell cycle, and only in appropriate subcellular locations. For example, Cdk2 is activated during the S and G2 phases of the cell cycle and is associated with cyclins A and E. The monomeric form of Cdk2 is inactive as a kinase, its conformation has multiple steric impediments to catalysis. Activation occurs in two steps, and is accompanied by conformational changes that remove the steric hindrances. The first step is the binding of a single Cdk2 monomer to a single molecule of cyclin. This allows for some enzymatic activity, but full activation of Cdk2 and all other known Cdks requires phosphorylation of a conserved threonine residue. Cak (for Cdk-activating kinase) has been shown to phosphorylate the threonine residue and activate Cdk2/cyclin complexes. Cak is itself a cyclin/Cdk complex consisting of cyclin H and Cdk7. Cyclin H is the regulatory subunit of the Cak enzyme, whereas Cdk7 is the catalytic subunit. Thus, Cak functions as a regulator of other cyclin/kinase complexes, suggesting that cyclin/kinase cascades may exist. Cdk7 migrates as a single band at a reduced molecular weight of 36-42 kDa. The MO-1 antibody recognizes human Cdk7.5 Recombinant human Cdk7 was used as an immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 40 kDa

Comments:

Related Products: ABIN967389, ABIN968533, ABIN968535

Synonyms: CAK, p34, p37, CAK1, MO15, STK1, CDKN7, p39MO15, Crk4, Cdkn7, AI323415, AI528512, C230069N13, CDK7, DmCDK7, DmMO15, cdk-7, dCdk7, p40[MO15], DmelCG3319, CG3319, MGC85821, zgc:85821, MGC140417, cak1, stk1, cdkn7, p39mo15, p40MO15

Application Details

Application Notes: Applications include western blot analysis (1-2 µg/ml). Other applications not routinely tested include immunoprecipitation (1 µg/ml cells), immunohistochemical staining of frozen tissue sections (1 µg/ml), and indirect immunofluorescence of tissue-cultured cells. The antibody is not recommended for immunohistochemical staining of paraffin-embedded tissue sections. HeLa human cervical carcinoma cells (ATCC CCL-2) or A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive immunoprecipitation and western blot controls.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Chromatin and Nuclear Signaling, Cell Cycle, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of Cdk7. Lysate from A-431 human epidermal carcinoma cells was probed with the MO-1 antibody (ABIN967506). Cdk7 is identified at ~40 kDa.

Frozen tissue section of human straified squamous carcinoma stained for Cdk7. A DAB chromogen and Hematoxylin counterstain is used. Cdk7 expression is identified in both normal epithelium (arrowhead) and tumor cells (arrows).

anti-Cyclin-Dependent Kinase 7 (CDK7) antibody (Image 3)

Publications

Product: Solomon: "The function(s) of CAK, the p34cdc2-activating kinase." in: Trends in biochemical sciences, Vol. 19, Issue 11, pp. 496-500, 1995 (PubMed).

Serizawa, Mäkelä, Conaway et al.: "Association of Cdk-activating kinase subunits with transcription factor TFIIH." in: Nature, Vol. 374, Issue 6519, pp. 280-2, 1995 (PubMed).

Tassan, Schultz, Bartek et al.: "Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase)." in: The Journal of cell biology, Vol. 127, Issue 2, pp. 467-78, 1994 (PubMed).

Fisher, Morgan: "A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase." in: Cell, Vol. 78, Issue 4, pp. 713-24, 1994 (PubMed).

Mäkelä, Tassan, Nigg et al.: "A cyclin associated with the CDK-activating kinase MO15." in: Nature, Vol. 371, Issue 6494, pp. 254-7, 1994 (PubMed).