Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|2 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Description||Clk (also referred to as Sty) is a mammalian protein kinase that phosphorylates both serine/threonine and tyrosine residues. It belongs to the LAMMER family of dual specificity kinases that also includes KNS1 (from Saccharomyces cerevisiae), AFC (from Arabidopsis thaliana), and Doa (from Drosophila melanogaster). Family members share a highly conserved motif in a subdomain, EHLAMMERILGPLP, of the kinase domain. This motif is not conserved among kinases in general and may be involved in directing substrate specificity. LAMMER kinases are thought to play important roles in the control of cellular growth and differentiation. In particular, Clk has been shown to phosphorylate members of the serine/arginine-rich (SR) family of RNA splicing factors, suggesting that it may play a role in RNA processing. G313-1 recognizes Clk1. Full-length Clk1 migrates at a reduced molecular weight of ~57 kDa. A full-length Clk1-fusion protein was used as immunogen.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||57 kDa|
Related Products: ABIN967389
|Application Notes||Applications include western blot analysis (1-2 µg/ml) and immunohistochemistry of tissue-cultured cells (1-5 µg/ml). MG-63 osteosarcoma cells (ATCC CRL-1427) are suggested as a positive control. In western blots, G313-1 detects an immunoreactive protein at 57 kDa representing full-length Clk1. A 40 kDa cross-reactive protein may also detected in MG-63 cells. Although the nature of this band has not been determined, it may be a Clk1 degradation product or differentially processed transcript. It is noted that the Clk gene has been reported to express several differentially processed transcripts. In immunohistochemistry, a speckled nuclear pattern, with nucleolar exclusion is observed. Not all cells are stained. In cells lacking nuclear staining, a weaker diffuse cytoplasmic staining may be observed.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
Duncan, Howell, Marius et al.: "Alternative splicing of STY, a nuclear dual specificity kinase." in: The Journal of biological chemistry, Vol. 270, Issue 37, pp. 21524-31, 1995 (PubMed).
Colwill, Pawson, Andrews et al.: "The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution." in: The EMBO journal, Vol. 15, Issue 2, pp. 265-75, 1996 (PubMed).