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Cytochrome C antibody

CYCS Reactivity: Human, Rat, Mouse, Pig WB, IP Host: Mouse Monoclonal 7H8-2C12 unconjugated
Catalog No. ABIN967529
  • Target See all Cytochrome C (CYCS) Antibodies
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Reactivity
    • 142
    • 112
    • 92
    • 45
    • 32
    • 24
    • 22
    • 19
    • 18
    • 16
    • 7
    • 6
    • 6
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Pig
    Host
    • 86
    • 70
    • 7
    • 1
    Mouse
    Clonality
    • 83
    • 80
    • 1
    Monoclonal
    Conjugate
    • 103
    • 12
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cytochrome C antibody is un-conjugated
    Application
    • 134
    • 48
    • 42
    • 39
    • 36
    • 33
    • 27
    • 23
    • 14
    • 14
    • 13
    • 13
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Brand
    BD Pharmingen™
    Cross-Reactivity
    Human, Mouse (Murine), Rat (Rattus), Horse (Equine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Synthetic Peptides of Pigeon Cytochrome C
    Clone
    7H8-2C12
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product CYCS Primary Antibody
  • Application Notes
    Applications include western blot analysis (1-2 µg/ml). Clone 7H8.2C12 is not useful for immunoprecipitation. However, clone 6H2.B4 is useful for immunoprecipitation of Cytochrome c.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4° C.
  • Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, (1996) (PubMed).

    Goshorn, Retzel, Jemmerson: "Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c." in: The Journal of biological chemistry, Vol. 266, Issue 4, pp. 2134-42, (1991) (PubMed).

  • Target
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Alternative Name
    Cytochrome c (CYCS Products)
    Synonyms
    CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody
    Background
    A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c, and d) according to spectrochemical characteristics, and there are five cytochromes between Coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2 - Cytochrome c reductase complex to the Cytochrome c oxidase complex. Recently it has been suggested that Cytochrome c may be required for cells to undergo apoptosis. Mitochondria may be involved in apoptosis by releasing Cytochrome c to the cytosol where it functions in the activation of Caspase 3 (CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated. In the cytoplasm Cytochrome C might exist in polymeric forms, appearing as a 58-60 kDa band by western blot analysis, rather than the monomeric form. Monomeric Cytochrome c migrates at a reduced molecular weight of 15 kDa.
    Clone 7H8.2C12 recognizes the denatured form of pigeon, horse, rat, mouse and human Cytochrome c. It does not recognize the native form of Cytochrome c. Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon Cytochrome C, based on competitive ELISA results. Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon Cytochrome c were used as immunogens. Clone 7H8.2C12 is used for western blot detection of Cytochrome c. Clone 6H2.B4, is used for immunoprecipitation. Cell lines P388D1 mouse lymphoma (ATCC CCL-46), HeLa human carcinoma (ATCC CCL-2), Jurkat T leukemia (ATCC TIB-152), and NIH/3T3 mouse fibroblast cells (ATCC CRL-1658) may be used as positive control cell lysates.
    This antibody is routinely tested by western blot analysis.
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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