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DNA-PK (p350) (AA 277-531) antibody

Details for Product No. ABIN967533
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Antigen
Epitope
AA 277-531
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunoprecipitation (IP)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967533
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Immunogen Human DNA-PK (p350) Recombinant Protein
Clone 4F10C5
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background The ability of mammalian cells to detect and repair DNA damage is essential in maintaining the structural integrity of their genes. Cells have evolved a number of repair pathways in order to repair different types of DNA lesions. DNA-dependent protein kinase (DNA-PK) is a serine-threonine protein kinase that is involved in DNA double-stranded break repair and variable-diversity-joining [V(D)J] recombination. To be active, DNA-PK must bind to double-stranded DNA containing broken ends, nicks, or single stranded gaps. Activated DNA-PK consists of three polypeptide components: Ku-80, Ku-70, and an ~350 kDa catalytic subunit (p350). First, Ku-80 and Ku-70 associate with each other to form a heterodimeric complex that binds to DNA breaks. By its interaction with DNA, Ku is thought to target p350 to sites of DNA damage. p350 then binds to the Ku-70/80 heterodimer (Ku) resulting in production of the active DNA-PK. Active DNA-PK phosphorylates a number of substrates in vitro, including proteins involved in the response of cells to DNA damage (p53, Ku, and replication protein A), and transcription factors (Sp1, Oct 1, Oct 2, SRF, Fos, and Jun). Clone 4F10C5 has been reported to recognize the 350 kDa catalytic subunit of DNA-PK.
Synonyms: DNA Dependent Protein Kinase
Molecular Weight 450-465 kDa
Application Notes For western blots, an extended transfer from a low percentage gel to the blot is necessary because the DNA-PK protein is so large. For example, a 4-12 hour transfer at 4°C may be needed for a 7.5% gel. HBL-100 human breast carcinoma cells (ATCC HTB-124) have been reported to be helpful as a positive control.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-DNA-PK (p350) (AA 277-531) antibody Western blot analysis for DNA-PK. HeLa cell lysates (Human cervical epitheloid carcinoma, ATCC CCL-2) were probed with the mouse anti- DNA-PK antibody at 0.5 µg/mL (lane 1), 1.0 µg/mL (lane 2) and 2.0 µg/mL (lane 3). Although the catalytic subunit has been reported to be 350 kDa, DNA-PK may be observable migrating in a range of 450-465 kDa. Processed forms of the catalytic subunit may also be observed running lower, such as between 115-180 kDa.
anti-DNA-PK (p350) (AA 277-531) antibody (2) anti-DNA-PK (p350) (AA 277-531) antibody (Image 2)
Product cited in: Peterson, Kurimasa, Oshimura et al.: "Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 8, pp. 3171-4, 1995 (PubMed).

Kirchgessner, Patil, Evans et al.: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Lees-Miller, Godbout, Chan et al.: "Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1183-5, 1995 (PubMed).

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