Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 335-469) antibody
|Synonyms||CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, FLJ17672, MGC78473, Mch5, Caspase-8, MGC92075, caspase-8, zgc:92075, casp8-A, xCaspase-8, CASP8, casp8, DKFZp469A1428|
Alternatives Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Recombinant Human Caspase-8|
Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of Caspase-8 contains an amino acid sequence, termed the death domain, that facilitates Caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing Caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of Caspase-8 have been isolated. Caspase-8 is produced as a proenzyme which upon receptor aggregation is proteolytically cleaved into 20 kDa and 10 kDa subunits. These subunits form a proteolytically active heterodimer capable of cleaving other Caspase family members, including PARP. Unlike other caspase family members, overexpression of FLICE is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the Caspase-3 protease inhibitor, CrmA. The B9-2 antibody recognizes an ~55 kDa band corresponding to human Caspase-8 (FLICE). A recombinant human Caspase-8 protein fragment corresponding to amino acids 335-469 was used as immunogen.
Synonyms: FLICE, MACH-1, Mch5
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||55 kDa|
Related Products: ABIN967389
|Application Notes||Applications include western blot analysis (1-2 µg/ml). Daudi B lymphoma cells (ATCC CCL 213) and HL-60 leukemia cells (ATCC CCL 240) are suggested as positive controls.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).
Thome, Schneider, Hofmann et al.: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997 (PubMed).
Thome, Martinon, Hofmann et al.: "Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 9962-8, 1999 (PubMed).