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Cyclin D1 antibody (CCND1) (full length)

Details for Product anti-CCND1 Antibody No. ABIN967539, Supplier: Log in to see
Antigen
  • ccnd1b
  • prad1
  • bcl1
  • d11s287e
  • u21b31
  • ccndbp1
  • ccnd1
  • ZHM
  • CCND1
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • ccnd1a
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
Alternatives
anti-Human Cyclin D1 antibody for Western Blotting
Epitope
full length
75
25
19
13
13
10
10
9
9
8
8
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
359
130
120
16
13
13
11
9
8
4
3
2
2
2
1
1
1
Host
Mouse
296
113
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Cyclin D1 antibody is un-conjugated
20
15
5
5
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
317
157
92
66
65
56
41
30
29
13
8
7
6
3
2
2
2
2
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Human Full-length Cyclin D1 Recombinant Protein
Clone DCS-6
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.
Molecular Weight 36 kDa
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle
Comment

Related Products: ABIN968585, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma, A...
 image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) anti-Cyclin D1 (CCND1) (full length) antibody (Image 2)
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) anti-Cyclin D1 (CCND1) (full length) antibody (Image 3)
Product cited in: Darzynkiewicz, Gong, Juan et al.: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997 (PubMed).

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).

Lukas, Pagano, Staskova et al.: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." in: Oncogene, Vol. 9, Issue 3, pp. 707-18, 1994 (PubMed).

de Boer, Schuuring, Dreef et al.: "Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma." in: Blood, Vol. 86, Issue 7, pp. 2715-23, 1995 (PubMed).