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TRADD antibody (TNFRSF1A-Associated Via Death Domain) (AA 14-62)

Details for Product anti-TRADD Antibody No. ABIN967541, Supplier: Log in to see
Antigen
  • 9130005N23Rik
  • AA930854
  • Hs.89862
  • TRADD
  • tradd
  • wu:fc59e02
  • zgc:103556
Alternatives
anti-Human TRADD antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 14-62
17
17
16
11
10
6
5
5
5
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
101
31
21
5
4
3
2
2
2
Host
Mouse
103
15
Clonality (Clone)
Monoclonal ()
Conjugate
This TRADD antibody is un-conjugated
5
5
2
2
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
94
50
46
26
24
20
16
11
4
2
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Human TRADD aa. 14-62
Clone B36-2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name TRADD (TRADD Antibody Abstract)
Background TRADD (TNF Receptor Associated Death domain) is an ~36 kDa protein that interacts specifically with the cytoplasmic domain of the type I TNF Receptor. Overexpression of the C-terminal 118 amino acids of TRADD, the death domain,” is sufficient to induce two major TNF-induced responses, apoptosis and activation of nuclear transcription factor, NF-kappaB. Evidence suggests that TRADD interacts with other signal molecules including TRAF1, TRAF2 and FADD, allowing recruitment of these molecules to the TNF receptor complex. A similar interaction has been demonstrated for TRADD and the serine/threonine kinase RIP. Coexpression of the ICE-specific protease inhibitor, CrmA, inhibits TRADD-induced apoptosis, but does not affect induction of NF-kappaB, suggesting that TRADD may serve to initiate distinct signal pathways. The B36-2 antibody recognizes an ~36 kDa band corresponding to human TRADD. A recombinant human TRADD protein fragment corresponding to amino acids 14-62 was used as immunogen.
Molecular Weight 36 kDa
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes Daudi B lymphoma cells (ATCC CRL-213) and Jurkat T cells (ATCC TIB-152) are suggested as positive controls.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-TRADD antibody (TNFRSF1A-Associated Via Death Domain) (AA 14-62) (ABIN967541) Western blot analysis of TRADD. Daudi lymphoma cell lysates were probed with anti-hum...
 image for anti-TRADD antibody (TNFRSF1A-Associated Via Death Domain) (AA 14-62) (ABIN967541) anti-TNFRSF1A-Associated Via Death Domain (TRADD) (AA 14-62) antibody (Image 2)
Product cited in: Hsu, Shu, Pan, Goeddel: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, 1996 (PubMed).

Hsu, Xiong, Goeddel: "The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation." in: Cell, Vol. 81, Issue 4, pp. 495-504, 1995 (PubMed).

Background publications Varfolomeev, Boldin, Goncharov, Wallach: "A potential mechanism of 'cross-talk' between the p55 tumor necrosis factor receptor and Fas/APO1: proteins binding to the death domains of the two receptors also bind to each other." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 1271-5, 1996 (PubMed).