TNFRSF1A-Associated Via Death Domain (TRADD) (AA 14-62) antibody
|Synonyms||Hs.89862, MGC11078, MGC103556, wu:fc59e02, zgc:103556, TRADD, MGC137615, tradd|
Alternatives Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Description||TRADD (TNF Receptor Associated Death domain) is an ~36 kDa protein that interacts specifically with the cytoplasmic domain of the type I TNF Receptor. Overexpression of the C-terminal 118 amino acids of TRADD, the death domain,” is sufficient to induce two major TNF-induced responses, apoptosis and activation of nuclear transcription factor, NF-kappaB. Evidence suggests that TRADD interacts with other signal molecules including TRAF1, TRAF2 and FADD, allowing recruitment of these molecules to the TNF receptor complex. A similar interaction has been demonstrated for TRADD and the serine/threonine kinase RIP. Coexpression of the ICE-specific protease inhibitor, CrmA, inhibits TRADD-induced apoptosis, but does not affect induction of NF-kappaB, suggesting that TRADD may serve to initiate distinct signal pathways. The B36-2 antibody recognizes an ~36 kDa band corresponding to human TRADD. A recombinant human TRADD protein fragment corresponding to amino acids 14-62 was used as immunogen.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||36 kDa|
|Application Notes||Daudi B lymphoma cells (ATCC CRL-213) and Jurkat T cells (ATCC TIB-152) are suggested as positive controls.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Cardiovascular, Atherosclerosis, Apoptosis/Necrosis|
|Restrictions||For Research Use only|
|Western blot analysis of TRADD. Daudi lymphoma cell lysates were probed with anti-human TRADD (clone B36-2). TRADD is identified as a 36 kDa band. anti-TNFRSF1A-Associated Via Death Domain (TRADD) (AA 14-62) antibody (Image 2)|
Hsu, Xiong, Goeddel: "The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation." in: Cell, Vol. 81, Issue 4, pp. 495-504, 1995 (PubMed).
Hsu, Shu, Pan et al.: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, 1996 (PubMed).
Varfolomeev, Boldin, Goncharov et al.: "A potential mechanism of "cross-talk" between the p55 tumor necrosis factor receptor and Fas/APO1: proteins binding to the death domains of the two receptors also bind to each other." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 1271-5, 1996 (PubMed).
|Hosts||Rabbit (62), Mouse (6), Goat (1)|
|Reactivities||Human (68), Mouse (Murine) (41), Rat (Rattus) (38), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (35), Immunofluorescence (IF) (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), ELISA (16), Immunoprecipitation (IP) (10), Immunohistochemistry (IHC) (6), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), PE (2), Biotin (1), CF (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer269 (5), C-Term (4), pSer274 (4), pSer299 (4), AA 1-312 (1), AA 172-192 (1), Center (1), Internal Region (1), Isoform 2 (1), pSer19 (1), pTyr174 (1)|