TNFRSF1A-Associated Via Death Domain (TRADD) (AA 14-62) antibody

Antigen: TNFRSF1A-Associated Via Death Domain (TRADD)

Synonyms: Hs.89862, MGC11078, MGC103556, wu:fc59e02, zgc:103556, TRADD, MGC137615, tradd

Binding Site: AA 14-62

Clonality: Monoclonal (B36-2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967541

Additional Information

Alternative name: TRADD

Immunogen: Human TRADD

Format: Liquid

Isotype: IgG1

Clone: B36-2

Description: TRADD (TNF Receptor Associated Death domain) is an ~36 kDa protein that interacts specifically with the cytoplasmic domain of the type I TNF Receptor. Overexpression of the C-terminal 118 amino acids of TRADD, the death domain,” is sufficient to induce two major TNF-induced responses, apoptosis and activation of nuclear transcription factor, NF-kappaB. Evidence suggests that TRADD interacts with other signal molecules including TRAF1, TRAF2 and FADD, allowing recruitment of these molecules to the TNF receptor complex. A similar interaction has been demonstrated for TRADD and the serine/threonine kinase RIP. Coexpression of the ICE-specific protease inhibitor, CrmA, inhibits TRADD-induced apoptosis, but does not affect induction of NF-kappaB, suggesting that TRADD may serve to initiate distinct signal pathways. The B36-2 antibody recognizes an ~36 kDa band corresponding to human TRADD. A recombinant human TRADD protein fragment corresponding to amino acids 14-62 was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 36 kDa

Application Details

Application Notes: Daudi B lymphoma cells (ATCC CRL-213) and Jurkat T cells (ATCC TIB-152) are suggested as positive controls.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Cardiovascular, Atherosclerosis, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of TRADD. Daudi lymphoma cell lysates were probed with anti-human TRADD (clone B36-2). TRADD is identified as a 36 kDa band.

anti-TNFRSF1A-Associated Via Death Domain (TRADD) (AA 14-62) antibody (Image 2)

Publications

Product: Hsu, Xiong, Goeddel: "The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation." in: Cell, Vol. 81, Issue 4, pp. 495-504, 1995 (PubMed).

Hsu, Shu, Pan et al.: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, 1996 (PubMed).

Varfolomeev, Boldin, Goncharov et al.: "A potential mechanism of "cross-talk" between the p55 tumor necrosis factor receptor and Fas/APO1: proteins binding to the death domains of the two receptors also bind to each other." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 1271-5, 1996 (PubMed).