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ID1 antibody (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein)

Details for Product anti-ID1 Antibody No. ABIN967545, Supplier: Log in to see
  • ID1
  • id6
  • cb108
  • wu:fb57e11
  • AI323524
  • D2Wsu140e
  • Idb1
  • bHLHb24
  • ID125A
  • ID
  • inhibitor of DNA binding 1
  • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
  • id1
  • ID1
  • Id1
Human, Mouse (Murine)
Clonality (Clone)
Monoclonal ()
This ID1 antibody is un-conjugated
Western Blotting (WB)
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Mouse Id1 (full-length)-GST Recombinant Protein
Clone B30-1
Isotype IgG2a, kappa
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Id1 (ID1 Antibody Abstract)
Background Id proteins were originally characterized as inhibitors of DNA binding and cell differentiation. Id1 through 4 contain an evolutionarily conserved helix-loop-helix (HLH) sequence which is critical for protein-protein interaction(s). Most HLH transcription factors contain a basic amino acid region adjacent to the HLH sequence, the bHLH sequence, which is responsible for DNA binding. bHLH transcription factors fall into 2 major groups designated class A factors, such as E2/2 and E47, and class B factors, such as MyoD, myogenin. In vitro studies demonstrate distinct interaction(s) between Id proteins and bHLH transcription factors. While Id proteins contain an HLH domain, they lack the basic region which is required for DNA binding. Therefore, Id proteins are negative regulators of transcription since complexes which contain them do not bind DNA. Id proteins are variably expressed throughout the cell cycle and are regulated by phosphorylation by cyclin-cdk complexes. Thus, Id proteins play an important role in transcriptional regulation of cell cycle related genes. Overexpression of Id1 can induce apoptosis in serum-starved fibroblasts and is correlated with cell cycle progression promoted by Id family members. Human Id1 has been reported to have a molecular weight of ~16-17 kDa. This antibody has been reported to recognize mouse Id1 and not to crossreact with either mouse Id2 or mouse Id3. This antibody is routinely tested by the western blot analysis.
Molecular Weight 45 kDa
Research Area Cancer, Angiogenesis, Hematopoietic Progenitors, Transcription Factors, CD Antigens

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Western Blotting (WB) image for anti-ID1 antibody (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein) (ABIN967545) Western blot analysis of recombinant human Id1. Purified mouse anti-Id1 antibody (clo...
 image for anti-ID1 antibody (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein) (ABIN967545) anti-Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) antibody (Image 2)
Western Blotting (WB) image for anti-ID1 antibody (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein) (ABIN967545) anti-Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) antibody (Image 3)
Product cited in: Norton, Atherton: "Coupling of cell growth control and apoptosis functions of Id proteins." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 2371-81, 1998 (PubMed).

Norton, Deed, Craggs, Sablitzky: "Id helix-loop-helix proteins in cell growth and differentiation." in: Trends in cell biology, Vol. 8, Issue 2, pp. 58-65, 1998 (PubMed).

Cooper, Brady, Bilia, Iscove, Quesenberry: "Expression of the Id family helix-loop-helix regulators during growth and development in the hematopoietic system." in: Blood, Vol. 89, Issue 9, pp. 3155-65, 1997 (PubMed).

Hara, Hall, Peters: "Cdk2-dependent phosphorylation of Id2 modulates activity of E2A-related transcription factors." in: The EMBO journal, Vol. 16, Issue 2, pp. 332-42, 1997 (PubMed).

Langlands, Yin, Anand, Prochownik: "Differential interactions of Id proteins with basic-helix-loop-helix transcription factors." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 19785-93, 1997 (PubMed).

Deed, Jasiok, Norton: "Nucleotide sequence of the cDNA encoding human helix-loop-helix Id-1 protein: identification of functionally conserved residues common to Id proteins." in: Biochimica et biophysica acta, Vol. 1219, Issue 1, pp. 160-2, 1994 (PubMed).

Riechmann, van Crüchten, Sablitzky: "The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3." in: Nucleic acids research, Vol. 22, Issue 5, pp. 749-55, 1994 (PubMed).

Kadesch: "Consequences of heteromeric interactions among helix-loop-helix proteins." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 4, Issue 1, pp. 49-55, 1993 (PubMed).