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Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody

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Synonyms ID3, MOR136-6, fa56g03, tdsubc_2g3, wu:fa56g03, ik:tdsubc_2g3, xx:tdsubc_2g3, heir-1, xid3, xidia, Hlh462, Idb3, bHLHb25, HEIR-1
Mouse (Murine)
(128), (25), (20), (2), (1), (1)
(74), (57)
Clonality (Clone)
Monoclonal ()
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(94), (45), (38), (27), (24), (23), (11), (9), (8), (4), (2), (1)
Pubmed 7 references available
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Quantity 0.1 mg
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Immunogen Fusion Protein Mouse Id3
Clone B72-1
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Id3 (ID3 Antibody Abstract)
Background Id proteins were originally characterized as inhibitors of DNA binding and cell differentiation. Id1 through 4 contain an evolutionarily conserved helix-loop-helix (HLH) sequence which is critical for protein-protein interaction(s). Most HLH transcription factors contain a basic amino acid region adjacent to the HLH sequence, the bHLH sequence, which is responsible for DNA binding. bHLH transcription factors fall into 2 major groups designated class A factors, e.g.,E2.2 and E47, and class B factors, e.g., MyoD and myogenin. In vitro studies demonstrate distinct interaction(s) between Id proteins and bHLH transcription factors. While Id proteins contain an HLH domain, they lack the basic region which is required for DNA binding. Therefore, Id proteins are negative regulators of transcription since complexes which contain them do not bind DNA. Id proteins are variably expressed throughout the cell cycle and are regulated by phosphorylation by cyclin-cdk complexes. Thus, Id proteins play an important role in transcriptional regulation of cell cycle related genes. Overexpression of Id3 can induce apoptosis in serum-starved fibroblasts by a mechanism which is correlated to Id3-mediated cell cycle progression and is rescued by over expression of the antiapoptotic proteins Bcl-2 and Bcl-XL. Clone B72-1 recognizes mouse Id3. It does not cross react with mouse Id1 or mouse Id2. A fusion protein containing full length mouse Id3 was used as immunogen. The antibody is routinely tested by western blotting of the Id3-fusion protein. The Id3-fusion protein is observed at 43 kDa (fusion protein MW ~28 kDa and Id3 MW is ~13 kDa).
Molecular Weight 13 kDa
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody (ABIN967547) Western blot analysis of purified mouse Id3-fusion protein using clone B72-1. Clone B...
 image for anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody (ABIN967547) anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody (Image 2)
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody (ABIN967547) anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) antibody (Image 3)
Product cited in: Norton, Deed, Craggs et al.: "Id helix-loop-helix proteins in cell growth and differentiation." in: Trends in cell biology, Vol. 8, Issue 2, pp. 58-65, 1998 (PubMed).

Norton, Atherton: "Coupling of cell growth control and apoptosis functions of Id proteins." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 2371-81, 1998 (PubMed).

Langlands, Yin, Anand et al.: "Differential interactions of Id proteins with basic-helix-loop-helix transcription factors." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 19785-93, 1997 (PubMed).

Cooper, Brady, Bilia et al.: "Expression of the Id family helix-loop-helix regulators during growth and development in the hematopoietic system." in: Blood, Vol. 89, Issue 9, pp. 3155-65, 1997 (PubMed).

Hara, Hall, Peters: "Cdk2-dependent phosphorylation of Id2 modulates activity of E2A-related transcription factors." in: The EMBO journal, Vol. 16, Issue 2, pp. 332-42, 1997 (PubMed).

Kadesch: "Consequences of heteromeric interactions among helix-loop-helix proteins." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 4, Issue 1, pp. 49-55, 1993 (PubMed).

Riechmann, van Crüchten, Sablitzky: "The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3." in: Nucleic acids research, Vol. 22, Issue 5, pp. 749-55, 1994 (PubMed).

Catalog No. ABIN967547
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