Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Description||The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor. E2F is now known to be a family of related factors, E2F-1 through E2F6 have been cloned. E2F proteins form heterodimers with the related proteins, DP-1 and -2. These complexes are regulated by their interaction with the retinoblastoma proteins (Rb, p107 and p130), which restrict E2F/DP complexes to the cytoplasm. E2F inhibitors are phosphorylated during the cell cycle. Phosphorylation of inhibitory proteins releases the E2F/DP complex to enter the nucleus, thus providing regulated activation of E2F responsive genes. E2F-DNA binding sites have been found in the promoter regions of genes important for growth regulation, e.g., c-myc, N-myc, cdc2 and cyclin A and within genes which are important for DNA synthesis. Activation of specific gene(s) may depend on the formation of distinct E2F complexes. Cotransfection studies demonstrate specific interactions between E2F and DP proteins, i.e., E2F-1 through -5 bind to DP-1, while E2F-4 binds to DP-2.4 The E2Fs also display differential binding to pRb family members. E2F gene products can promote cell cycle aprogression and proliferation. E2F-1, -2 and -3 are transcriptional activators which by themselves can drive cells through S phase, and can override G1 block, E2F-4 and -5 require interaction with DP to do so. E2F-6 is unique in that it is not regulated by pRb family members and instead acts to repress transcription activation by other E2F members. E2F-3 migrates at a molecular weight of 66 kDa by SDS/PAGE. Clone TFE31 recognizes an epitope between amino acids 1 through 132 of human E2F-3. It does not cross-react with other E2F proteins. The specificity of the antibody was verified by immunoprecipitation of in vitro translated E2F-3 and by western blot analysis of cell extracts.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||66 kDa|
Related Products: ABIN967389
|Application Notes||Applications include western blot analysis (1-2 µg/ml). Daudi Burkitt lymphoma cells (ATCC CCL-213) are recommended as a positive control for western blot analysis. TFE31 may also be used for immunoprecipitation of in vitro translated E2F-2 protein.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
|Western blot analysis of human E2F-3. Lysates from Daudi Burkitt lymphoma cells were probed with anti E2F-3 (clone TFE31, ABIN967549). E2F-3 is detected at ~66 kDa. anti-E2F3 antibody (Image 2)|
Nevins: "E2F: a link between the Rb tumor suppressor protein and viral oncoproteins." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 424-9, 1992 (PubMed).
Lukas, Petersen, Holm et al.: "Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1047-57, 1996 (PubMed).
Magae, Wu, Illenye et al.: "Nuclear localization of DP and E2F transcription factors by heterodimeric partners and retinoblastoma protein family members." in: Journal of cell science, Vol. 109 ( Pt 7), Issue 25, pp. 1717-26, 1997 (PubMed).
Sladek: "E2F transcription factor action, regulation and possible role in human cancer." in: Cell proliferation, Vol. 30, Issue 3-4, pp. 97-105, 1997 (PubMed).
Trimarchi, Fairchild, Verona et al.: "E2F-6, a member of the E2F family that can behave as a transcriptional repressor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 6, pp. 2850-5, 1998 (PubMed).
|Hosts||Rabbit (34), Mouse (9)|
|Reactivities||Human (39), Mouse (Murine) (25), Rat (Rattus) (22), Cow (Bovine) (20), Dog (Canine) (20), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (23), Western Blotting (WB) (17), ELISA (14), Gel Shift (GS) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Electrophoretic Mobility-Shift Assay (EMSA) (2), Immunocytochemistry (ICC) (2), Immunohistochemistry (IHC) (2), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (2), FITC (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (18), AA 70-82 (1), C-Term (1), Center (1), Internal Region,AA 150-180 (1)|