conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody

Antigen: conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)

Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053

Clonality: Monoclonal (B78-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967550

Additional Information

Alternative name: IKK alpha

Immunogen: Human IKKalpha

Format: Liquid

Isotype: IgG2b

Clone: B78-1

Description: The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2). IKKalpha and IKKbeta are serine/threonine kinases which share approximately 50% sequence identity. In addition to their kinase domains, these proteins each contain leucine zipper and helix-loop-helix regions which facilitate their interaction with each other as well as interaction(s) with other proteins within the IKK complex. Both IKKalpha and IKKbeta phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. IKKalpha and IKKbeta become activated themselves following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKalpha migrates at a molecular weight of 85 kD in SDS-PAGE. Clone B78-1 recognizes human IKKalpha. It does not cross-react with IKKbeta. Full length, recombinant human IKKalpha was used as immunogen. The reactivity of B78-1 was verified by immunoprecipitation and western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 85 kDa

Comments:

Related Products: ABIN967389, ABIN968535

Application Details

Application Notes: Applications include western blot analysis (1-2 µg/ml). Immunoprecipitation (1-2 µg of antibody per one million cells or 1-2 µg of antibody for 300 µg of total protein) was also reported for clone B78-1. The following human cell lines are suggested as positive controls: A-204 (Rhabdomyosarcoma, ATCC HTB-82), RD (Rhabdomyosarcoma, ATCC CCL-136), HeLa (cervical carcinoma, ATCC CCL-2), Daudi (Burkitt lymphoma, ATCC CCL-213) and 293 transformed embryonic kidney cells (ATCC CRL-1573).

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of IKKalpha. Lane 1, lysate from Daubi B lymphoma cellswas probed with anti-IKKalpha (clone B78-1, ABIN967550). Lane 2, mouse IgG2bkappa, isotype control.

anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 2)

Publications

Product: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Woronicz, Gao, Cao et al.: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).

Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).