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CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase)

Details for Product anti-CHUK Antibody No. ABIN967550, Supplier: Log in to see
Antigen
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
Alternatives
anti-Human CHUK antibody for Western Blotting
Reactivity
Human
291
96
71
2
2
Host
Mouse
249
47
10
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CHUK antibody is un-conjugated
14
10
10
8
8
8
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
266
134
83
38
37
32
29
20
10
6
4
2
2
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Human IKKalpha
Clone B78-1
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2). IKKalpha and IKKbeta are serine/threonine kinases which share approximately 50% sequence identity. In addition to their kinase domains, these proteins each contain leucine zipper and helix-loop-helix regions which facilitate their interaction with each other as well as interaction(s) with other proteins within the IKK complex. Both IKKalpha and IKKbeta phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. IKKalpha and IKKbeta become activated themselves following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKalpha migrates at a molecular weight of 85 kD in SDS-PAGE. Clone B78-1 recognizes human IKKalpha. It does not cross-react with IKKbeta. Full length, recombinant human IKKalpha was used as immunogen. The reactivity of B78-1 was verified by immunoprecipitation and western blot analysis.
Molecular Weight 85 kDa
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Applications include western blot analysis (1-2 µg/ml). Immunoprecipitation (1-2 µg of antibody per one million cells or 1-2 µg of antibody for 300 µg of total protein) was also reported for clone B78-1. The following human cell lines are suggested as positive controls: A-204 (Rhabdomyosarcoma, ATCC HTB-82), RD (Rhabdomyosarcoma, ATCC CCL-136), HeLa (cervical carcinoma, ATCC CCL-2), Daudi (Burkitt lymphoma, ATCC CCL-213) and 293 transformed embryonic kidney cells (ATCC CRL-1573).
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) Western blot analysis of IKKalpha. Lane 1, lysate from Daubi B lymphoma cellswas prob...
 image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 2)
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 3)
Product cited in: Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).

Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Woronicz, Gao, Cao et al.: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).