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Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (AA 25-42)

Details for Product anti-CASP7 Antibody No. ABIN967551, Supplier: Login to see New
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Synonyms xCaspase-7, caspase-7, caspase7, cmh-1, ice-lap3, mch3, CASP7, AI314680, CMH-1, ICE-IAP3, Mch3, mCASP-7, CASP-7, ICE-LAP3, LICE2, MCH3, Caspase-7, MCH3/SCA-2, SCA-2
AA 25-42
(34), (28), (12), (9), (7), (6), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(252), (87), (63), (8), (7), (5), (4), (4), (3), (1), (1), (1)
(205), (55), (4)
Clonality (Clone)
Monoclonal ()
This Caspase 7 antibody is un-conjugated
(12), (10), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunoprecipitation (IP), Western Blotting (WB)
(215), (86), (79), (54), (31), (17), (12), (10), (7), (4), (4), (3), (2), (1)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human Capase-7 aa. 25-42
Clone B94-1
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Caspase-7 is a member of a family of cysteine proteases which play a critical role in the induction of apoptosis. Caspase-7 is structurally and functionally most similar to caspase-3. These proteases are expressed as proenzymes which are processed into large and small subunits that associate to form the active enzyme. Caspase-7 may also exist as alternative splice forms, i.e., Mch3alpha and Mch3beta. A novel interaction between Mch3alpha and the 17 kDa subunit of caspase-3 has been demonstrated in vitro, producing a heteromeric enzyme which is proteolytically active. Active caspase-7 can move to the mitochondrial fraction of cells undergoing Fas-mediated apoptosis, whereas active caspase-3 is primarily cytosolic. Caspase-7 is activated by caspase-31 and by granzyme B. Active caspase-7 can cleave the nuclear substrate PARP1 as well as the sterol regulatory element-binding protein 1 (SREBP-1). Caspase-7 migrates at a molecular weight of 35 kDa in SDS/PAGE. Clone B94-1 recognizes the 35 kDa and 17 kDa form human caspase-7. The specificity of the antibody was verified by immunoprecipitation and western blot analysis of active, recombinant human caspase-7 and of endogenous caspase-7 in cell lysates. A synthetic peptide corresponding to amino acids 25-42 of human caspase-7 was used as immunogen.
Synonyms: Mch3
Molecular Weight 35 kDa (pro caspase-7), 17 kDa (active capase-7)
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes This antibody is routinely tested by western blot analysis (0.5-1.0 µg/ml). Other applications not routinely tested include immunoprecipitation (1-2 µg/1 x 10^6 cells). 293 embryonic kidney (ATCC CRL-1673) are recommended as a positive control. Jurkat T cell leukemia (ATCC TIB-152) and Daudi Burkitt lymphoma (ATCC CCL-213) cell lines may also be used for these applications.

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (AA 25-42) (ABIN967551) Western blot analysis of caspase-7. Lysates from 293 embryonic kidney cells were prob...
 image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (AA 25-42) (ABIN967551) anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody (Image 2)
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (AA 25-42) (ABIN967551) anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody (Image 3)
Product cited in: Chandler, Cohen, MacFarlane: "Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10815-8, 1998 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Chinnaiyan, Hanna, Orth et al.: "Cytotoxic T-cell-derived granzyme B activates the apoptotic protease ICE-LAP3." in: Current biology : CB, Vol. 6, Issue 7, pp. 897-9, 1997 (PubMed).

Duan, Chinnaiyan, Hudson et al.: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).

Lippke, Gu, Sarnecki et al.: "Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 1825-8, 1996 (PubMed).

Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).

Catalog No. ABIN967551
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