Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody

Details for Product No. ABIN967551
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Antigen
Synonyms CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, caspase7, cmh-1, ice-lap3, mch3, CASP7
Epitope
AA 25-42
(19), (16), (9), (8), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(163), (84), (65), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Host
Mouse
(138), (39), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(151), (72), (51), (49), (20), (20), (15), (8), (7), (4), (3), (2), (1), (1)
Pubmed 6 references available
Catalog no. ABIN967551
Quantity 0.1 mg
Price
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Immunogen Human Capase-7
Clone B94-1
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Caspase-7
Background Caspase-7 is a member of a family of cysteine proteases which play a critical role in the induction of apoptosis. Caspase-7 is structurally and functionally most similar to caspase-3. These proteases are expressed as proenzymes which are processed into large and small subunits that associate to form the active enzyme. Caspase-7 may also exist as alternative splice forms, i.e., Mch3alpha and Mch3beta. A novel interaction between Mch3alpha and the 17 kDa subunit of caspase-3 has been demonstrated in vitro, producing a heteromeric enzyme which is proteolytically active. Active caspase-7 can move to the mitochondrial fraction of cells undergoing Fas-mediated apoptosis, whereas active caspase-3 is primarily cytosolic. Caspase-7 is activated by caspase-31 and by granzyme B. Active caspase-7 can cleave the nuclear substrate PARP1 as well as the sterol regulatory element-binding protein 1 (SREBP-1). Caspase-7 migrates at a molecular weight of 35 kDa in SDS/PAGE. Clone B94-1 recognizes the 35 kDa and 17 kDa form human caspase-7. The specificity of the antibody was verified by immunoprecipitation and western blot analysis of active, recombinant human caspase-7 and of endogenous caspase-7 in cell lysates. A synthetic peptide corresponding to amino acids 25-42 of human caspase-7 was used as immunogen.
Synonyms: Mch3
Molecular Weight 35 kDa (pro caspase-7), 17 kDa (active capase-7)
Research Area Apoptosis/Necrosis
Application Notes This antibody is routinely tested by western blot analysis (0.5-1.0 µg/ml). Other applications not routinely tested include immunoprecipitation (1-2 µg/1 x 10^6 cells). 293 embryonic kidney (ATCC CRL-1673) are recommended as a positive control. Jurkat T cell leukemia (ATCC TIB-152) and Daudi Burkitt lymphoma (ATCC CCL-213) cell lines may also be used for these applications.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody Western blot analysis of caspase-7. Lysates from 293 embryonic kidney cells were probed with anti-human caspase-7 (clone B94-1). Caspase-7 was detected at ~35 kDa.
anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody (2) anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 25-42) antibody (Image 2)
Product cited in: Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).

Lippke, Gu, Sarnecki et al.: "Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 1825-8, 1996 (PubMed).

Duan, Chinnaiyan, Hudson et al.: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).

Chinnaiyan, Hanna, Orth et al.: "Cytotoxic T-cell-derived granzyme B activates the apoptotic protease ICE-LAP3." in: Current biology : CB, Vol. 6, Issue 7, pp. 897-9, 1997 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Chandler, Cohen, MacFarlane: "Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10815-8, 1998 (PubMed).

Hosts (138), (39), (2)
Reactivities (163), (84), (65), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Applications (151), (72), (51), (49), (20), (20), (15), (8), (7), (4), (3), (2), (1), (1)
Conjugates (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (19), (16), (9), (8), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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