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Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (AA 271-285)

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Synonyms xCaspase-6, mch2, caspase-6, zgc:112960, CASP6, casp6, Mch2, mCASP-6, MCH2
AA 271-285
(58), (41), (34), (23), (17), (15), (13), (13), (11), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(284), (82), (79), (15), (3), (2)
(281), (19), (18)
Clonality (Clone)
Monoclonal ()
This Caspase 6 antibody is un-conjugated
(19), (18), (14), (11), (11), (11), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(262), (154), (109), (57), (35), (26), (25), (16), (9), (6), (5), (5), (1)
Pubmed 5 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human Caspase-6 aa. 271-285
Clone B93-4
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-6 (CASP6 Antibody Abstract)
Background The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. ICE (interleukin-1ß converting enzyme) was the first member of this family to be discovered following a search for human proteins with homology to ced-3, a cell death gene identified in C. elegans. Caspase has been adopted as a root name for all family members based on the properties of these enzymes. The c reflects a cysteine protease mechanism and aspase refers to their ability to cleave after aspartic acid residues. Caspases are synthesized as inactive proenzymes that are processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms consist of large (17-22 kDa) and small (10-12 kDa) subunits which 34kDa associate to form an active enzyme. Caspase-6 exists as two isoforms, designated Mch2alpha (caspase-6) and Mch2ß, a proteolytically inactive isoform which lacks half of the large subunit.3 Caspase-6 is most closely related to caspase-3, and can be proteolytically cleaved/activated by caspase-3 into large (p18) and small (p11) subunits. Similarly, caspase-3 has been shown to be activated by caspase-6, suggesting that these proteases may generate an amplification cycle during apoptosis. Other proteolytical targets of Western blot analysis of caspase-6. In lane 1, active caspase-6 include lamin A and a cytosolic nuclease which translocates to the Daudi B cell lysate was probed with anti-nucleus to promote DNA fragmentation. Caspase-6 has also been shown to cleave Fak (focal adhesion kinase), a non-receptor protein tyrosine kinase that transduces cell Clone B93-4 identifies full length caspase-6 at survival and proliferation signals from contact sites between the cell surface and extracellular matrix. In this instance, caspase-6 cleavage inhibits the anti-apoptotic function of Fak. Therefore, the protease activity of caspase-6 plays both activating and inhibitory roles in apoptotic pathways. Caspase-6 is observed at ~34 kDa in SDS-PAGE. The B93-4 clone recognizes human caspase-6. The antibody detects both full length caspase-6 (34 kDa), as well as the p11 subunit of the active enzyme. A synthetic peptide sequence corresponding to amino acids 271-285 (KKQVPCFASMLTKK) of human caspase-6 was used as immunogen.
Molecular Weight 34/11 kDa
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Applications include western blot analysis (2 µg/ml). Recombinant human caspase-6 or 293 human embryonic kidney cells (ATCC CRL-1573) are suggested as positive controls for this application.

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (AA 271-285) (ABIN967553) Western blot analysis of caspase-6. In lane 1, Daudi B cell lysate was probed with an...
 image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (AA 271-285) (ABIN967553) anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 271-285) antibody (Image 2)
Western Blotting (WB) image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (AA 271-285) (ABIN967553) anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (AA 271-285) antibody (Image 3)
Product cited in: Cryns, Yuan: "Proteases to die for." in: Genes & development, Vol. 12, Issue 11, pp. 1551-70, 1998 (PubMed).

Mitamura, Ikawa, Mizuno et al.: "Cytosolic nuclease activated by caspase-3 and inhibited by DFF-45." in: Biochemical and biophysical research communications, Vol. 243, Issue 2, pp. 480-4, 1998 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Fernandes-Alnemri, Litwack, Alnemri: "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family." in: Cancer research, Vol. 55, Issue 13, pp. 2737-42, 1995 (PubMed).

Catalog No. ABIN967553
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