Alternatives Western Blotting (WB)
|4 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Description||14-3-3 proteins are a family of small, evolutionarily conserved proteins which display various wide-ranging functions. 14-3-3 isoforms are found in all vertebrates, invertebrates, plants and fungi. These have been classified in mammalian species as typical (gamma, eta, beta/alpha, zeta/delta, t) or atypical (sigma and epsilon) isoforms, based on their distribution and apparent functional roles. While 14-3-3 isoforms are expressed at ubiquitous, low levels in most human tissues, all 14-3-3 isoforms (with the exception of sigma) are primarily expressed in neurons. 14-3-3 proteins are highly enriched in areas of neuronal death associated with Alzheimer's disease and have been reported as a marker for this disease. 14-3-3sigma, which inhibits the activity of Cdc25C. In response to DNA damage, Cdc25C binds to 14-3-3sigma, which inhibits the activity of Cdc25C and results in G2 cell cycle arrest. In addition, 14-3-3 may play a role in apoptosis via binding to the pro-apoptotic molecule, BAD. 14-3-3 isoforms have molecular masses of ~30 kDa on SDS-PAGE. C23-1 reacts with human 14-3-3. Full length, recombinant human 14-3-3 was used as immunogen. The C23-1 clone recognizes a 14-3-3 isoform of ~31 kDa on SDS-PAGE. The identity of this isoform has not been characterized.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||30 kDa|
Related Products: ABIN967389
|Application Notes||Clone C23-1 is recommended for western blot analysis (1-2 µg/ml). MOLT-4 lymphoblastic leukemia (ATCC CRL-1582) or Jurkat T cells (ATCC TIB-152) are suggested as positive controls for this application.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
|Western blot analysis of 14-3-3. Lysate from Eahy human endothelial cells was probed with anti-14-3-3 (ABIN967554) was titrated between 1 myg/ml and 0.04 myg/ml (lanes 1-3), and identifies 14-3-3 at ~30 kDa. anti-14.03.2003 antibody (Image 2)|
Layfield, Fergusson, Aitken et al.: "Neurofibrillary tangles of Alzheimer's disease brains contain 14-3-3 proteins." in: Neuroscience letters, Vol. 209, Issue 1, pp. 57-60, 1996 (PubMed).
Zha, Harada, Yang et al.: "Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L)" in: Cell, Vol. 87, Issue 4, pp. 619-28, 1997 (PubMed).
Skoulakis, Davis: "14-3-3 proteins in neuronal development and function." in: Molecular neurobiology, Vol. 16, Issue 3, pp. 269-84, 1998 (PubMed).
Hermeking, Lengauer, Polyak et al.: "14-3-3 sigma is a p53-regulated inhibitor of G2/M progression." in: Molecular cell, Vol. 1, Issue 1, pp. 3-11, 1998 (PubMed).