Cyclin A1 (CCNA1) (AA 46-93) antibody

Details for Product No. ABIN967557
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Antigen
Synonyms fd14f08, wu:fd14f08, wu:fi20d12, zgc:111869, MGC81965, cyclin-A1, CCNA1, MGC132235, cyclin-A1-2, Cyclin-A1, ccna1
Epitope
AA 46-93
(8), (6), (5), (3), (1), (1), (1), (1), (1)
Reactivity
Human
(55), (44), (31), (12), (12), (12)
Host
Mouse
(57), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(51), (35), (18), (10), (7), (6), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967557
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Immunogen Human cyclin A1
Clone B88-2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin A1
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27[Kip1]. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, tumor suppressor genes, nucleolin and others. Cyclin A plays a regulatory role during both S-phase and G2/M transitions through its association with cdk2 and cdk1, respectively. A related cyclin, designated cyclin A1, has also been identified. Cyclin A1 shares 48% sequence identity with cyclin A. Yeast two hybrid analysis has shown that, like cyclin A, cyclin A1 also forms a complex with cdk2. Cyclin A1 does not appear to bind cdk1, cdk2 or cdk5. In contrast to cyclin A, cyclin A1 appears to be predominantly expressed in myeloid leukemia cells, however mRNA analysis has suggested that low levels of gene expression may be found in many other hematopoietic and non-hematopoietic cell types. Human cyclin A1 is observed at ~ 65 kD by SDS-PAGE. B88-2 reacts with human cyclin A1. It does not cross-react with cyclin A. A polypeptide fragment containing amino acids 46-93 of human cyclin A1 was used as immunogen.
Molecular Weight 65 kDa
Research Area Cancer, Cell Cycle
Application Notes Clone B88-2 may be used for western blot analysis (8 µg/ml). THP-1 human monocyte cells (ATCC TIB202) are suggested as a positive control for this application.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin A1 (CCNA1) (AA 46-93) antibody Western blot analysis of cyclin A1 in THP-1 human monocyte lysate. Anti-human cycllin A1 identifies cyclin A1 as an ~ 65 kD band.
anti-Cyclin A1 (CCNA1) (AA 46-93) antibody (2) anti-Cyclin A1 (CCNA1) (AA 46-93) antibody (Image 2)
Product cited in: Gong, Bhatia, Traganos et al.: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995 (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993 (PubMed).

Yang, Morosetti, Koeffler: "Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines." in: Cancer research, Vol. 57, Issue 5, pp. 913-20, 1997 (PubMed).

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