Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG)

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Clonality: Monoclonal (C73-1794)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967570

Additional Information

Alternative name: IKK gamma

Immunogen: Recombinant Human IKKgamma

Format: Liquid

Isotype: IgG1

Clone: C73-1794

Description: The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF- kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKgamma forms migrate as a doublet of ~50-55 kDa in SDS-PAGE. Clone C73-1794 recognizes human IKKgamma. Purified, recombinant human IKKgamma was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 50/55 kDa doublet

Comments:

Related Products: ABIN967389

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Application Details

Application Notes: The C73-1794 antibody may be used for western blot analysis (2 µg/ml). Jurkat human T cells (ATCC TIB-152) and SK-N-NH human neuroblastoma cells (ATCC HTB-11) are suggested as positive controls for this application.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of IKKgamma in SK-N-SH human neuroblastoma cell lysates. Lane 1. anti-IKKgamma. Lane 2, a mouse IgG1 isotope control.

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (Image 2)

Publications

Product: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).

Rothwarf, Zandi, Natoli et al.: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).