IKBKG antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma)

Details for Product anti-IKBKG Antibody No. ABIN967570, Supplier: Log in to see
Antigen
  • nemo
  • fj33c07
  • wu:fj33c07
  • zgc:113492
  • MGC97885
  • IKBKG
  • IKBKG_1
  • IKBKG_2
  • CG16910
  • DmIKK-gamma
  • DmIKKgamma
  • Dmel\\CG16910
  • Dmikkgamma
  • IKK
  • IKK-gamma
  • IKK[[gamma]]
  • IKKg
  • IKKgamma
  • KEY
  • Kenny
  • Key
  • dIKK
  • dIKK-gamma
  • dmIKKgamma
  • AMCBX1
  • FIP-3
  • FIP3
  • Fip3p
  • IP
  • IP1
  • IP2
  • IPD2
  • NEMO
  • ZC2HC9
  • Nemo
  • 1110037D23Rik
  • AI848108
  • AI851264
  • AW124339
  • IKK[g]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • kenny
  • inhibitor of kappaB kinase gamma
  • ikbkg
  • IKBKG
  • LOC782205
  • IKBKG_1
  • key
  • Ikbkg
Alternatives
anti-Human IKBKG antibody for ELISA
Reactivity
Human
315
114
81
10
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3
2
2
2
1
Host
Mouse
227
95
2
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IKBKG antibody is un-conjugated
11
9
8
6
6
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4
4
4
4
4
4
3
3
3
3
2
2
2
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2
Application
Western Blotting (WB)
250
98
69
55
51
36
23
20
6
5
5
3
2
1
1
Options
Supplier
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Brand BD Pharmingen™
Immunogen Recombinant Human IKKgamma
Clone C73
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IKK gamma (IKBKG Antibody Abstract)
Background The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF- kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKgamma forms migrate as a doublet of ~50-55 kDa in SDS-PAGE. Clone C73-1794 recognizes human IKKgamma. Purified, recombinant human IKKgamma was used as immunogen.
Molecular Weight 50/55 kDa doublet
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades
Application Notes The C73-1794 antibody may be used for western blot analysis (2 µg/ml). Jurkat human T cells (ATCC TIB-152) and SK-N-NH human neuroblastoma cells (ATCC HTB-11) are suggested as positive controls for this application.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-IKBKG antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma) (ABIN967570) Western blot analysis of IKKgamma in SK-N-SH human neuroblastoma cell lysates. Lane 1...
 image for anti-IKBKG antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma) (ABIN967570) anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (Image 2)
Product cited in: Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).