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Ly-6G and Ly-6C antibody

Details for Product No. ABIN967573, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
4
Host
Rat
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
Application
Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
4
2
2
2
2
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Not Reported
Clone RB6-8C5
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the lineage cocktail used in studies of hematopoietic lineages. The mAb 1A8 specifically recognizes Ly-6G, but not Ly-6C. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Gr-1
Application Notes For immunohistochemistry, we recommend using purified RB6-8C5 antibody in our special formulation for IHC.
Comment

Related Products: ABIN967573

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Product cited in: Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, 1996

Rakhmilevich: "Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes." in: Journal of leukocyte biology, Vol. 57, Issue 6, pp. 827-31, 1995

Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, 1994

Czuprynski, Brown, Maroushek, Wagner, Steinberg: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, 1994

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, 1993

Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." in: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, 1992

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, 1991

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, 1989

Background publications Tumpey, Chen, Oakes, Lausch: "Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea." in: Journal of virology, Vol. 70, Issue 2, pp. 898-904, 1996

Stoppacciaro, Melani, Parenza, Mastracchio, Bassi, Baroni, Parmiani, Colombo: "Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma." in: The Journal of experimental medicine, Vol. 178, Issue 1, pp. 151-61, 1993

Brummer, Sugar, Stevens: "Immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro." in: Journal of leukocyte biology, Vol. 36, Issue 4, pp. 505-20, 1984