Ezrin (EZR) (pTyr353) antibody

Details for Product No. ABIN967575
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Antigen
Synonyms CVIL, CVL, VIL2, Vil2, AW146364, R75297, p81, ATVLN2, T11A7.16, T11A7_16, villin 2, ezr, vil2, wu:fc05c03, wu:fj31d06, cvil, cvl
Epitope
pTyr353
(32), (24), (22), (21), (17), (13), (12), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(249), (176), (152), (52), (40), (36), (36), (12), (2), (2)
Host
Mouse
(220), (23), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(202), (97), (50), (50), (31), (16), (6), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN967575
Quantity 0.1 mg
Price
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Immunogen Phosphorylated Human Ezrin Peptide
Clone I66-386
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Ezrin
Background Ezrin is a member of the ERM (ezrin-radixin-moesin) family of proteins that function as crosslinkers between the actin cytoskeleton and the plasma membrane. Ezrin is a substrate for the tyrosine kinase epidermal (EGF) and hepatocyte growth factor (HGF) receptors. Upon EGF or HGF stimulation, ezrin is phosphorylated on tyrosine 353 (Y353), which is required for its interaction with phosphatidylinositol 3 kinase p85 subunit and activation of Akt.
The I66-386 monoclonal antibody recognizes the phosphorylated Y353 of human ezrin.
Molecular Weight 80 kDa
Research Area Cancer, Cytoskeleton, Microfilaments
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Ezrin (EZR) (pTyr353) antibody Western blot analysis of Ezrin (pY353). Human K562 cells were either left untreated (lanes 1-3) or treated with pervanadate (lanes 4-6). Blots were probed with anti-Ezrin (pY353) antibody at concentrations of 0.125 (lanes 1 and 4), 0.063 (lanes 2 and 5), and 0.032 ug/ml (lanes 3 and 6). Ezrin (pY353) is identified as a band of ~80 kDa
Product cited in: Gautreau, Poullet, Louvard et al.: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, 1999 (PubMed).

Hosts (220), (23), (10)
Reactivities (249), (176), (152), (52), (40), (36), (36), (12), (2), (2)
Applications (202), (97), (50), (50), (31), (16), (6), (4), (3), (2), (1), (1), (1), (1)
Conjugates (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (32), (24), (22), (21), (17), (13), (12), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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