Western blot analysis of c-Cbl (pY774) in human T leukemia. Lysates from control (ABIN968537, left panel) and Pervanadate-treated (ABIN968656, right panel) Jurkat cells were probed with purified mouse anti-c-Cbl (pY774) monoclonal antibody at concentrations of 1.0, 0.5, and 0.25 myg/ml (Lanes 1, 2, and 3, respectively). c-Cbl (pY774) is identified as a band of 120 kDa in the treated cells.
c-Cbl (pY774) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with Retrievagen A buffer, the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel). The tissue sections were stained with purified mouse anti-c-Cbl (pY774) with Hematoxylin counterstaining. Original magnification: 20X.