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CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr774)

Details for Product anti-CBL Antibody No. ABIN967577, Supplier: Log in to see
Antigen
  • 4732447J05Rik
  • Cbl-2
  • c-Cbl
  • cbl
  • C-CBL
  • CBL2
  • FRA11B
  • NSLL
  • RNF55
  • c-cbl-like
  • RGD1561386
Alternatives
anti-Human CBL antibody for Western Blotting
Epitope
pTyr774
46
40
25
23
17
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
246
133
127
6
4
4
4
1
1
1
Host
Mouse
200
34
16
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CBL antibody is un-conjugated
7
7
7
7
6
6
5
5
5
5
5
5
4
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
164
68
66
56
51
30
18
13
5
5
4
3
3
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Phosphorylated Human c-Cbl Peptide
Clone 29-c
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name c-Cbl (CBL Antibody Abstract)
Background Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700, 731, and 774 (Y774) by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y774 site interacts with the SH2 domain of the CRK adapter protein. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 29/c-Cbl monoclonal antibody recognizes the Y774-phosphorylated form of human c-Cbl.
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway
Comment

Related Products: ABIN967389, ABIN968537, ABIN968656

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr774) (ABIN967577) Western blot analysis of c-Cbl (pY774) in human T leukemia. Lysates from control (ABI...
 image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr774) (ABIN967577) c-Cbl (pY774) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadat...
Product cited in: Tsygankov, Teckchandani, Feshchenko et al.: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, 2001 (PubMed).

Thien, Langdon: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, 2001 (PubMed).