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p53 antibody (Tumor Protein P53) (Ser46)

Details for Product anti-TP53 Antibody No. ABIN967583, Supplier: Log in to see
Antigen
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Alternatives
anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Ser46, pSer46
156
103
87
67
65
64
55
52
51
50
46
46
41
39
30
30
27
25
25
22
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2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
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1
1
1
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1
1
1
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Reactivity
Human
2069
387
321
120
101
89
56
54
29
23
19
15
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1239
928
7
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This p53 antibody is un-conjugated
68
61
49
45
31
31
23
23
17
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6
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3
1
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Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
1655
732
564
492
442
421
397
230
194
156
53
38
35
17
16
12
11
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11
9
8
4
3
3
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2
2
2
1
1
1
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1
Supplier
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Brand BD Pharmingen™
Immunogen Phosphorylated peptide corresponding to the region including Serine 46 of p53
Clone I117-1091
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p53 (TP53 Antibody Abstract)
Background The p53 protein is critical to regulation of normal cell growth and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. MDM2 mediates feedback inhibition of p53, which is prevented by phosphorylations of p53 amino-terminal serines and threonines. UV radiation-induced phosphorylation of Serine 46 (S46) is mediated by homeodomain-interacting protein kinase-2 (HIPK2) and is involved in the promotion of apoptosis. Other stress stimuli mediate S46 phosphorylation in p53 via as yet unidentified kinase(s).
The I117-1091 mAb recognizes p53 phosphorylated at S46.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (Ser46) (ABIN967583) Western blot analysis of p53 (pS46). Lysates from control (First Panel) and UV-irradi...
 image for anti-p53 antibody (Tumor Protein P53) (Ser46) (ABIN967583) p53 (pS46) staining on human colon carcinoma. The colon carcinoma tissue was fixed in...
 image for anti-p53 antibody (Tumor Protein P53) (Ser46) (ABIN967583) Cells treated with a phosphatase to eliminate all phosphorylation
Product cited in: Bode, Dong: "Post-translational modification of p53 in tumorigenesis." in: Nature reviews. Cancer, Vol. 4, Issue 10, pp. 793-805, 2004 (PubMed).

Di Stefano, Blandino, Sacchi, Soddu, DOrazi: "HIPK2 neutralizes MDM2 inhibition rescuing p53 transcriptional activity and apoptotic function." in: Oncogene, Vol. 23, Issue 30, pp. 5185-92, 2004 (PubMed).

DOrazi, Cecchinelli, Bruno, Manni, Higashimoto, Saito, Gostissa, Coen, Marchetti, Del Sal, Piaggio, Fanciulli, Appella, Soddu: "Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis." in: Nature cell biology, Vol. 4, Issue 1, pp. 11-9, 2002 (PubMed).

Hofmann, Möller, Sirma, Zentgraf, Taya, Dröge, Will, Schmitz: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." in: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, 2002 (PubMed).

Saito, Goodarzi, Higashimoto, Noda, Lees-Miller, Appella, Anderson: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, 2002 (PubMed).

Background publications De Milito, Aleman, Marenzi, Sonnerborg, Fuchs, Zazzi, Chiodi, D: "Plasma levels of soluble CD27: a simple marker to monitor immune activation during potent antiretroviral therapy in HIV-1-infected subjects." in: Clinical and experimental immunology, Vol. 127, Issue 3, pp. 486-94, 2002 (PubMed).